College of Food Science and Engineering, Jilin Agricultural University, Changchun, Jilin 130118, China.
College of Life Science, Jilin Agricultural University, Changchun, Jilin 130118, China.
Int J Biol Macromol. 2022 May 31;208:453-462. doi: 10.1016/j.ijbiomac.2022.03.134. Epub 2022 Mar 24.
Hosta ventricosa is a plant that can be used for medicine and diet. It has been proven to have anti-inflammatory, antibacterial and antitumor activities, and one of its main constituents is polysaccharides. However, studies on polysaccharides of Hosta ventricosa are limited, and their physiological activities have not been clarified. Therefore, isolation, purification and characterization of Hosta ventricosa root polysaccharides (HVRPp-1) were performed in this research. Furthermore, the effect of HVRPp-1 on tert-butyl hydroperoxide (t-BHP)-induced oxidative damage in HepG2 cells was investigated in vitro. The results showed that HVRPp-1 is a nonhomogeneous polysaccharide that could protect HepG2 cells from oxidative damage through the C-Jun N-terminal kinase (JNK)/nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway. In conclusion, this research proved the antioxidant mechanism of HVRPp-1 for the first time, providing a reliable theoretical basis for basic research on Hosta ventricosa polysaccharides and the possibility of their application in functional foods.
玉簪是一种可药用和食用的植物。已证实其具有抗炎、抗菌和抗肿瘤活性，其主要成分之一是多糖。然而，对玉簪多糖的研究有限，其生理活性尚未阐明。因此，本研究对玉簪根多糖（HVRPp-1）进行了分离、纯化和特性分析。此外，还在体外研究了 HVRPp-1 对 t-BHP 诱导的 HepG2 细胞氧化损伤的影响。结果表明，HVRPp-1 是一种不均一多糖，可通过 C-Jun N-末端激酶（JNK）/核因子红细胞 2 相关因子 2（Nrf2）信号通路保护 HepG2 细胞免受氧化损伤。总之，本研究首次证明了 HVRPp-1 的抗氧化机制，为玉簪多糖的基础研究及其在功能性食品中的应用提供了可靠的理论依据。
