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用于检测尼罗河三角洲地区绵羊中裂谷热病毒抗体的血清学检测

Serological tests for detecting Rift Valley fever viral antibodies in sheep from the Nile Delta.

作者信息

Scott R M, Feinsod F M, Allam I H, Ksiazek T G, Peters C J, Botros B A, Darwish M A

出版信息

J Clin Microbiol. 1986 Oct;24(4):612-4. doi: 10.1128/jcm.24.4.612-614.1986.

Abstract

To determine the accuracy of serological methods in detecting Rift Valley fever (RVF) viral antibodies, we examined serum samples obtained from 418 sheep in the Nile Delta by using five tests. The plaque reduction neutralization test (PRNT) was considered the standard serological method against which the four other tests were compared. Twenty-four serum samples had RVF viral antibodies detected by PRNT. Hemagglutination inhibition and enzyme-linked immunosorbent assay antibodies to RVF virus were also present in the same 24 serum samples. Indirect immunofluorescence was less sensitive in comparison with PRNT, and complement fixation was the least sensitive. These results extend observations made with laboratory animals to a large field-collected group of Egyptian sheep.

摘要

为确定血清学方法检测裂谷热(RVF)病毒抗体的准确性,我们使用五种检测方法对从尼罗河三角洲418只绵羊采集的血清样本进行了检测。蚀斑减少中和试验(PRNT)被视为标准血清学方法,用于与其他四种检测方法进行比较。通过PRNT检测发现24份血清样本含有RVF病毒抗体。血凝抑制试验以及针对RVF病毒的酶联免疫吸附测定抗体也存在于这24份相同的血清样本中。与PRNT相比,间接免疫荧光法的敏感性较低,而补体结合试验的敏感性最低。这些结果将在实验动物身上的观察结果扩展到了一大群从埃及绵羊身上采集的野外样本。

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