State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, P. R. China.
School of Food Science and Technology, Jiangnan University, Wuxi, P. R. China.
J Sci Food Agric. 2022 Oct;102(13):5618-5627. doi: 10.1002/jsfa.11897. Epub 2022 Aug 3.
Isolation of lysozyme from egg white (EW) using ion exchange resin adsorption method generates large quantities of lysozyme-free egg white (LFEW) with poor gelling property. To maximize the applications of LFEW, the effect of Lactiplantibacillus plantarum fermentation on the gel properties of LFEW was investigated in this study.
The fermentation efficiency of LFEW with lysozyme removed was significantly improved, and the sugar removal rate (2 g kg Lactiplantibacillus plantarum, 37 °C, 7 h) was more than 90%. Removal of lysozyme resulted in increased stability and surface hydrophobicity of EW. After Lactiplantibacillus plantarum fermentation, the stability of EW decreased, and the average particle size and surface hydrophobicity increased. In addition, by comparing the gel properties of EW and LFEW before and after fermentation at different pH, it was found that the hardness, elasticity, and water holding capacity (WHC) of EW gel increased significantly. The removal of lysozyme effectively improved the WHC and springiness of the EW gel and promoted the formation of a denser network structure with smaller pores. After Lactiplantibacillus plantarum fermentation treatment, LFEW gel hardness decreased, with loose and porous network structure, no browning occurred after autoclaving.
This study provided the direction and theoretical basis for producing a fermented LFEW gel with pleasing texture and appearance. © 2022 Society of Chemical Industry.
采用离子交换树脂吸附法从蛋清(EW)中分离溶菌酶会产生大量溶菌酶-free 蛋清(LFEW),其凝胶性能较差。为了最大限度地利用 LFEW,本研究考察了植物乳杆菌发酵对 LFEW 凝胶性能的影响。
去除溶菌酶的 LFEW 的发酵效率显著提高,糖去除率(2 g/kg 植物乳杆菌,37°C,7 h)超过 90%。去除溶菌酶可提高 EW 的稳定性和表面疏水性。植物乳杆菌发酵后,EW 的稳定性降低,平均粒径和表面疏水性增加。此外,通过比较发酵前后不同 pH 值下 EW 和 LFEW 的凝胶性能,发现 EW 凝胶的硬度、弹性和持水力(WHC)显著增加。溶菌酶的去除有效地提高了 EW 凝胶的 WHC 和弹性,促进了更致密的网络结构的形成,具有较小的孔。经植物乳杆菌发酵处理后,LFEW 凝胶硬度降低,网络结构疏松多孔,高压灭菌后无褐变现象。
本研究为生产口感和外观令人愉悦的发酵 LFEW 凝胶提供了方向和理论基础。© 2022 化学工业协会。