College of Food Science, Northeast Agricultural University, Harbin 150030, P.R. China.
College of Engineering, Northeast Agricultural University, Harbin 150030, P.R. China.
Poult Sci. 2024 Aug;103(8):103876. doi: 10.1016/j.psj.2024.103876. Epub 2024 May 17.
The aim of this study was to elucidate the different effects and difference mechanism of gelling properties among egg white (EW) treated with different heating intensities and the composite addition of rhamnolipid and soybean lecithin. Particle size analyzer, potentiometric analyzer, surface hydrophobicity method, and Fourier transform infrared spectroscopy techniques were used to determine the physicochemical properties and molecular structure, respectively. Low-field nuclear magnetic resonance, magnetic resonance imaging, texture profile analysis, and scanning electron microscopy techniques were used to analyze the gelling properties and gel structure, respectively. And we illuminate the different mechanisms in the gelling properties of the EW with various treatments and key internal factors that play important roles in improving gelling properties by establishing the link between the gelling properties and relevant characteristics by mixed effects model and visual network analysis. The results indicate raising the content of rhamnolipid decreased the migration of immobilized water in the EW gel and the free water content. At the heating intensities of 55 °C/3.5, 65 °C/2.5, and 67 °C/1.5 min, with an increase in rhamnolipid, the gel's cohesiveness, gumminess, and chewiness gradually increased. The mixed effects model indicated that heating intensities and composite ratios have a 2-way interaction on zeta potential, the relaxation time of bound water (T), the content of bound water (P), the content of immobilized water (P), and fractal dimension (d) attributes (P < 0.05). The visual network analysis showed that the protein solubility, the relaxation time of immobilized water (T), surface hydrophobicity, zeta potential, average particle size (d) and the relaxation time of free water (T) are critical contributors to the different gelling properties of EW subjected to various treatments and the improvement of gelling properties. This study will provide theoretical guidance for the development of egg white products and the expansion of egg white's application scope in the egg product processing industry.
本研究旨在阐明不同加热强度处理的蛋清(EW)和鼠李糖脂与大豆卵磷脂复合添加的胶凝特性的不同影响和差异机制。分别采用粒径分析仪、电位分析仪、表面疏水性法和傅里叶变换红外光谱技术测定理化性质和分子结构。采用低场核磁共振、磁共振成像、质构剖面分析和扫描电子显微镜技术分别分析凝胶特性和凝胶结构。通过混合效应模型和可视化网络分析,建立凝胶特性与相关特性之间的联系,阐明不同处理方式下 EW 凝胶特性的不同机制和对改善凝胶特性起重要作用的关键内部因素。结果表明,提高鼠李糖脂的含量会降低 EW 凝胶中固定水的迁移和自由水的含量。在 55°C/3.5、65°C/2.5 和 67°C/1.5 min 的加热强度下,随着鼠李糖脂含量的增加,凝胶的粘性、胶粘性和咀嚼性逐渐增加。混合效应模型表明,加热强度和复合比例对ζ电位、结合水的弛豫时间(T)、结合水含量(P)、固定水含量(P)和分形维数(d)属性有 2 -way 交互作用(P<0.05)。可视化网络分析表明,蛋白质溶解度、固定水的弛豫时间(T)、表面疏水性、ζ电位、平均粒径(d)和自由水的弛豫时间(T)是不同处理方式下 EW 不同胶凝特性和胶凝特性改善的关键贡献因素。本研究将为蛋清产品的开发和蛋清在蛋品加工行业应用范围的扩大提供理论指导。