Sasaki Akira
Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki, 305-8566 Japan.
Biophys Rev. 2021 Nov 16;14(1):33-39. doi: 10.1007/s12551-021-00871-0. eCollection 2022 Feb.
Fluorescence microscopy is a broadly used technique within a variety of different fields, including life sciences and medical research, and its quantitative aspects are becoming emphasized. The challenge now lies in improving the accuracy and precision of the measurements obtained from fluorescence microscopy. Improving these will facilitate the comparison of results between different instruments/institutions and therefore ensure the reproducibility of results. In this review, recent standardization procedures, including the benchmarking of the instrument performance and the standardization of the image itself, as well as the reference materials for calibration are summarized, and an overview of the advances in fluorescence microscopy standardization and the current limitations are presented. A procedure for the comparison of the image data obtained using different instruments, by different analysts and/or at different times, should be developed to improve the standardization of this data. The standardization of image data would lead to the development of new applications of fluorescence microscopy not only in academic research but also in regulatory science.
荧光显微镜是一种广泛应用于包括生命科学和医学研究在内的各种不同领域的技术，并且其定量方面正受到越来越多的重视。现在的挑战在于提高从荧光显微镜获得的测量的准确性和精确性。提高这些将有助于不同仪器/机构之间结果的比较，从而确保结果的可重复性。在这篇综述中，总结了最近的标准化程序，包括仪器性能的基准测试、图像本身的标准化以及校准的参考材料，并概述了荧光显微镜标准化的进展和当前的局限性。应该开发一种程序，用于比较由不同分析师在不同时间使用不同仪器获得的图像数据，以提高这些数据的标准化。图像数据的标准化不仅将推动荧光显微镜在学术研究中的新应用，还将推动其在监管科学中的新应用。
