Strobel E S, Gay R E, Greenberg P L
Int J Cell Cloning. 1986 Sep;4(5):341-56. doi: 10.1002/stem.5530040506.
Utilizing long-term in vitro culture techniques, we characterized the cellular composition and functional attributes of the human in vitro bone marrow stromal microenvironment. Morphologic, specific cytochemical and immunologic methods demonstrated that the marrow stromal adherent layer (AL) reached confluency at two to three weeks, and was comprised of 60%-70% fibroblastic cells, 10%-20% endothelial cells, 10%-20% monocyte/macrophages and 5%-10% fat-laden adherent cells. These proportions of cell types persisted for at least three months concomitant with proliferation of CFU-gm and BFU-e. In contrast, umbilical cord blood cells did not form a stromal AL despite persistence of hemopoietic progenitor cell proliferation. These findings provide a basis for improved understanding of cellular interactions regulating hemopoiesis.
利用长期体外培养技术，我们对人体外骨髓基质微环境的细胞组成和功能特性进行了表征。形态学、特异性细胞化学和免疫学方法表明，骨髓基质贴壁层（AL）在两到三周时达到汇合，由60%-70%的成纤维细胞、10%-20%的内皮细胞、10%-20%的单核细胞/巨噬细胞和5%-10%的含脂贴壁细胞组成。这些细胞类型的比例至少持续三个月，同时伴有CFU-gm和BFU-e的增殖。相比之下，尽管造血祖细胞持续增殖，但脐带血细胞并未形成基质AL。这些发现为更好地理解调节造血的细胞间相互作用提供了基础。
