Tuomanen E, Cozens R, Tosch W, Zak O, Tomasz A
J Gen Microbiol. 1986 May;132(5):1297-304. doi: 10.1099/00221287-132-5-1297.
Nongrowing bacteria evade the bactericidal activity of beta-lactam antibiotics. We sought to determine if slow growth rate also alters bactericidal activity. The bactericidal activity of two beta-lactams on Escherichia coli grown in glucose limited chemostats was compared for generation times ranging from 0.7 to 12 h. The degree of killing varied with drug structure and with E. coli strain. However, all killing rates were a constant function of the bacterial generation time: slowly growing bacteria became progressively more phenotypically tolerant to beta-lactam antibiotics as the generation time was extended.
非生长型细菌可逃避β-内酰胺类抗生素的杀菌活性。我们试图确定生长速率缓慢是否也会改变杀菌活性。比较了两种β-内酰胺类药物对在葡萄糖限制恒化器中生长的大肠杆菌的杀菌活性，其代时范围为0.7至12小时。杀菌程度因药物结构和大肠杆菌菌株而异。然而，所有的杀菌速率都是细菌代时的恒定函数：随着代时延长，生长缓慢的细菌对β-内酰胺类抗生素的表型耐受性逐渐增强。
