Biochemistry and Molecular Biology Laboratory of the Federal Institute of Education, Science, and Technology of Rio Grande do Sul - Sertão Campus, City of Sertão, State of Rio Grande do Sul, Brazil; Graduate Program in Pharmacology, Universidade Federal de Santa Maria, Av. Roraima, 1000, Cidade Universitária, Camobi, Santa Maria, RS 97105-900, Brazil.
Graduate Program in Environmental Science and Technology, Federal University of Fronteira Sul (UFFS) - Erechim Campus, City of Erechim, State of Rio Grande do Sul, Brazil.
Environ Toxicol Pharmacol. 2022 May;92:103857. doi: 10.1016/j.etap.2022.103857. Epub 2022 Mar 25.
Estrogen is considered to be an endocrine disrupter and is becoming increasingly more prevalent in the daily life of humans. In some cases, estrogen is not fully metabolized by organisms and may be excreted in either its original form or in organic complex forms, into water residue systems reaching concentrations of 0.05 ng.L to 75 ng.L. However, estrogen 17α-ethinylestradiol (EE2), which is used in oral contraceptives, is very difficult to remove from water. Here, we evaluated whether the synthetic hormone, EE2, affects the nervous system and the behavior of adult zebrafish. We established a range of concentrations (0.05, 0.5, 5, 50, and 75 ng.L), in addition to the control, to evaluate the effect of this compound and its bioaccumulation in zebrafish tissues. Here we show that EE2 bioaccumulates in fish and can change its behavior with an increased time in the upper zone (novel tank test) and far from the shoal segment (social preference test), demonstrating a clear anxiolytic pattern. The anxiolytic effect of EE2 can be harmful as it can affect the stress response of the species. The results presented herein reinforce the idea that the presence of EE2 in environmental water can be dangerous for non-target animals.
雌激素被认为是一种内分泌干扰物，在人类的日常生活中越来越普遍。在某些情况下，雌激素不能被生物体完全代谢，可能以其原始形式或有机复合物的形式排泄到水残留系统中，浓度达到 0.05ng.L 至 75ng.L。然而，用于口服避孕药的合成激素 17α-乙炔基雌二醇（EE2）很难从水中去除。在这里，我们评估了合成激素 EE2 是否会影响成年斑马鱼的神经系统和行为。我们建立了一系列浓度（0.05、0.5、5、50 和 75ng.L），除了对照，以评估这种化合物及其在斑马鱼组织中的生物累积的影响。在这里，我们表明 EE2 在鱼类体内积累，并能改变其行为，在上面区域（新鱼缸测试）和远离鱼群区域（社会偏好测试）的时间增加，表现出明显的抗焦虑模式。EE2 的抗焦虑作用可能是有害的，因为它会影响物种的应激反应。本文的结果强化了这样一种观点，即在环境水中存在 EE2 可能对非目标动物是危险的。
