Ma Yu, Gao Jinbo, Guo Hongning
Department of Stomatology, Tianjin Third Central Hospital, Tianjin 300170, China.
J Oncol. 2022 Mar 18;2022:8719542. doi: 10.1155/2022/8719542. eCollection 2022.
To investigate the effects of microRNA-23a (miR-23a-3p) and Runx2 on malignant progression of oral cancer cells and their possible molecular mechanisms.
Fluorescence quantitative PCR (qPCR) was used to detect the expression of miR-23a-3p and Runx2 in human oral squamous cell carcinoma tissues and paracancerous tissues. The dual luciferase reporter assay was used to evaluate the targeted regulation of miR-23a-3p on Runx2. A subcutaneous xenograft model was established to investigate the tumor-suppressive effect of miR-23a-3p. Cells were transfected with miR-23a-3p mimics and negative control NC. CCK-8 assay, EDU assay, Transwell assay, and clone formation assay were used to detect malignant evolution of cells. Western blotting was used to detect the expression of Runx2, PTEN, and PI3K/Akt. The cells were simultaneously transfected with miR-23a-3p mimics and Runx2 to detect the malignant evolution of cells.
The expression of miR-23a-3p was downregulated in oral squamous cell carcinoma tissues, while the expression of Runx2 was upregulated. Overexpression of miR-23a-3p or inhibition of Runx2 inhibited the malignant progression of oral squamous cell carcinoma CAL-27 and TSCCA. Overexpression of miR-23a-3p inhibits the growth of oral cancer tumors. miR-23a-3p inhibits the PTEN/PI3K/Akt signaling pathway through Runx2. Overexpression of Runx2 reverses the tumor-suppressive effect of miR-23a-3p.
miR-23a-3p can inhibit the PI3K/Akt signaling pathway by targeting Runx2 and inhibit the malignant evolution of oral cancer.
探讨微小RNA-23a（miR-23a-3p）和Runx2对口腔癌细胞恶性进展的影响及其可能的分子机制。
采用荧光定量聚合酶链反应（qPCR）检测miR-23a-3p和Runx2在人口腔鳞状细胞癌组织及癌旁组织中的表达。采用双荧光素酶报告基因检测法评估miR-23a-3p对Runx2的靶向调控作用。建立皮下异种移植模型以研究miR-23a-3p的抑瘤作用。将细胞分别转染miR-23a-3p模拟物和阴性对照NC。采用CCK-8法、EDU法、Transwell法和克隆形成试验检测细胞的恶性演变。采用蛋白质免疫印迹法检测Runx2、PTEN和PI3K/Akt的表达。将细胞同时转染miR-23a-3p模拟物和Runx2以检测细胞的恶性演变。
miR-23a-3p在口腔鳞状细胞癌组织中的表达下调，而Runx2的表达上调。miR-23a-3p的过表达或Runx2的抑制可抑制口腔鳞状细胞癌CAL-27和TSCCA的恶性进展。miR-23a-3p的过表达可抑制口腔癌肿瘤的生长。miR-23a-3p通过Runx2抑制PTEN/PI3K/Akt信号通路。Runx2的过表达可逆转miR-23a-3p的抑瘤作用。
miR-23a-3p可通过靶向Runx2抑制PI3K/Akt信号通路，从而抑制口腔癌的恶性演变。
