Zhu Xing-Long, Lu Li-Jie, Wu Qing-Hua, Wei Dan, Yan Yu-Hang, Ren Chao-Xiang, Pei Jin
State Key Laboratory of Southwestern Chinese Medicine Resources Chengdu 611137, China School of Pharmacy,Chengdu University of Traditional Chinese Medicine Chengdu 611137, China.
Zhongguo Zhong Yao Za Zhi. 2022 Mar;47(5):1262-1272. doi: 10.19540/j.cnki.cjcmm.20211110.302.
In this study, we employed Q Exactive to determine the main differential metabolites of Magnoliae Officinalis Cortex du-ring the "sweating" process. Further, we quantified the color parameters and determined the activities of polyphenol oxidase(PPO), peroxidase(POD), and tyrosinase of Magnoliae Officinalis Cortex during the "sweating" process. Gray correlation analysis was performed for the color, chemical composition, and enzyme activity to reveal the effect of enzymatic reaction on the color of Magnoliae Officinalis Cortex during the "sweating" process. Magnoliae Officinalis Cortex sweating in different manners showed similar metabolite changes. The primary metabolites that changed significantly included amino acids, nucleotides, and sugars, and the secondary metabolites with significant changes were phenols and phenylpropanoids. Despite the different sweating methods, eleven compounds were commonly up-regulated, including L-glutamic acid, acetylarginine, hypoxanthine, and xanthine; six compounds were commonly down-re-gulated, including L-arginine, L-aspartic acid, and phenylalanine. The brightness value(L*), red-green value(a), and yellow-blue value(b~) of Magnoliae Officinalis Cortex kept decreasing during the "sweating" process. The changes in the activities of PPO and POD during sweating were consistent with those in the color parameter values. The gray correlation analysis demonstrated that the main differential metabolites such as amino acids and phenols were closely related to the color parameters L*, a* and b~*; POD was correlated with amino acids and phenols; PPO had strong correlation with phenols. The results indicated that the color change of Magnoliae Officinalis Cortex during "sweating" was closely related to the reactions of enzymes dominated by PPO and POD. The study analyzed the correlations among the main differential metabolites, color parameters, and enzyme activities of Magnoliae Officinalis Cortex in the "sweating" process. It reveals the common law of material changes and ascertains the relationship between color changes and enzymatic reactions of Magnoliae Officinalis Cortex during "sweating". Therefore, this study provides a reference for studying the "sweating" mechanism of Magnoliae Officinalis Cortex and is of great significance to guarantee the quality of Magnoliae Officinalis Cortex.
在本研究中，我们采用Q Exactive来确定厚朴在“发汗”过程中的主要差异代谢物。此外，我们对厚朴在“发汗”过程中的颜色参数进行了量化，并测定了多酚氧化酶（PPO）、过氧化物酶（POD）和酪氨酸酶的活性。对颜色、化学成分和酶活性进行灰色关联分析，以揭示“发汗”过程中酶促反应对厚朴颜色的影响。不同发汗方式的厚朴显示出相似的代谢物变化。显著变化的主要代谢物包括氨基酸、核苷酸和糖类，显著变化的次生代谢物为酚类和苯丙烷类。尽管发汗方法不同，但有11种化合物普遍上调，包括L-谷氨酸、乙酰精氨酸、次黄嘌呤和黄嘌呤；6种化合物普遍下调，包括L-精氨酸、L-天冬氨酸和苯丙氨酸。厚朴在“发汗”过程中亮度值（L*）、红绿色值（a）和黄蓝色值（b~）持续下降。发汗过程中PPO和POD活性的变化与颜色参数值的变化一致。灰色关联分析表明，氨基酸和酚类等主要差异代谢物与颜色参数L*、a*和b~*密切相关；POD与氨基酸和酚类相关；PPO与酚类有很强的相关性。结果表明，厚朴在“发汗”过程中的颜色变化与以PPO和POD为主的酶促反应密切相关。本研究分析了厚朴在“发汗”过程中主要差异代谢物、颜色参数和酶活性之间的相关性。揭示了物质变化的共同规律，明确了厚朴在“发汗”过程中颜色变化与酶促反应的关系。因此，本研究为研究厚朴的“发汗”机制提供了参考，对保证厚朴质量具有重要意义。
