Discipline of Clinical Anatomy, School of Laboratory Medicine & Medical Sciences, Nelson R Mandela School of Medicine, University of KwaZulu-Natal, Durban, South Africa.
Discipline of Physiology, School of Laboratory Medicine & Medical Sciences, College of Health Sciences, Westville Campus, University of KwaZulu-Natal, Durban, South Africa.
Artif Cells Nanomed Biotechnol. 2022 Dec;50(1):71-80. doi: 10.1080/21691401.2022.2042009.
Reproductive derangement and metabolic disorders in human immunodeficiency virus (HIV) infected persons require a nanoparticle delivery system to convey antiretroviral drugs to the anatomical sanctuary such as testis. This study investigated the effects of tenofovir disoproxil fumarate (TDF) loaded silver nanoparticles (AgNPs) on the testicular oxidative stress, inflammatory cytokines and histology in male diabetic rats. Thirty-six Sprague-Dawley rats weighing 230 ± 20 g were randomly divided into diabetic and non-diabetic groups ( = 18). Diabetes was induced using the fructose-streptozotocin (Frt-STZ) rat model. Both groups were further divided into three ( = 6) and administered distilled water, TDF, or TDF-AgNP. Results obtained with the TDF-AgNP administration showed a significant increase ( < .05) in the reduced glutathione and catalase levels. Tumour necrosis factor-alpha and interleukin 6 were reduced in diabetic rats administered TDF-AgNP. More so, administration of TDF-AgNP to diabetic rats improved testicular histoarchitecture in diabetic rats. In addition, diabetic rats administered TDF-AgNP showed a significant reduction ( < .05) in blood glucose levels. TDF-AgNP to diabetic rats enhanced testicular antioxidant enzyme, reduced testicular inflammation, and alleviated structural derangements in the testis. Thus, the application of AgNP to deliver TDF may alleviate testicular toxicity and subsequently cater for neglected reproductive dysfunction during the management of HIV infection.
人类免疫缺陷病毒 (HIV) 感染者的生殖功能障碍和代谢紊乱需要一种纳米颗粒输送系统将抗逆转录病毒药物递送到睾丸等解剖避难所。本研究探讨了负载富马酸替诺福韦二吡呋酯 (TDF) 的银纳米颗粒 (AgNP) 对雄性糖尿病大鼠睾丸氧化应激、炎症细胞因子和组织学的影响。36 只体重为 230±20g 的 Sprague-Dawley 大鼠随机分为糖尿病组和非糖尿病组（每组 n=18）。使用果糖-链脲佐菌素 (Frt-STZ) 大鼠模型诱导糖尿病。两组均进一步分为三组（每组 n=6），分别给予蒸馏水、TDF 或 TDF-AgNP。TDF-AgNP 给药组的结果显示，还原型谷胱甘肽和过氧化氢酶水平显著升高（ < .05）。糖尿病大鼠中肿瘤坏死因子-α和白细胞介素 6 减少。此外，给予 TDF-AgNP 可改善糖尿病大鼠的睾丸组织形态。此外，给予 TDF-AgNP 的糖尿病大鼠的血糖水平显著降低（ < .05）。TDF-AgNP 改善了糖尿病大鼠的睾丸抗氧化酶，减轻了睾丸炎症，并缓解了睾丸结构紊乱。因此，AgNP 用于递送 TDF 可能会减轻睾丸毒性，并在治疗 HIV 感染期间满足被忽视的生殖功能障碍的需求。
