School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, PR China.
Scientific Instrument Center, Shanxi University, Taiyuan 030006, PR China.
Anal Methods. 2022 Apr 7;14(14):1462-1470. doi: 10.1039/d1ay02176a.
A new phenazine-imidazole based Schiff base (PIS) fluorescent probe has been developed for the ratiometric detection of Cd ions in aqueous media at physiological pH. PIS upon binding with Cd ions shows red shifted fluorescence and thereby, permits ratiometric detection of Cd(II) ions. A detection limit down to 2.10 × 10 M was determined for Cd quantitation. Also, the accompanying apparent fluorescence color change (from yellow to orange red) is noticeable to the naked eye under a UV-lamp. The sensing mechanism could be attributed to the 1 : 1 PIS-Cd complexation, followed by extension of the conjugation due to better planarity and modulation of the charge transfer efficiency in the probe. This was complemented by solvatochromism and density functional theory calculations. Furthermore, PIS was used to detect Cd in Oxya chinensis cells, zebrafish larvae and live tissues of under a fluorescence microscope, showing great potential in analyzing living biosystems.
一种新型的吩嗪-咪唑基席夫碱（PIS）荧光探针已被开发用于在生理 pH 值的水相中对 Cd 离子进行比率检测。PIS 与 Cd 离子结合后会显示出红移荧光，从而允许对 Cd(II)离子进行比率检测。Cd 的定量检测下限低至 2.10×10-7 M。此外，在紫外灯下，伴随的明显荧光颜色变化（从黄色变为橙红色）肉眼可见。传感机制可归因于 1:1 PIS-Cd 络合，随后由于探针中更好的平面性和电荷转移效率的调制而导致共轭延伸。这得到了溶剂化变色和密度泛函理论计算的补充。此外，PIS 还被用于在荧光显微镜下检测中华稻蝗细胞、斑马鱼幼虫和活组织中的 Cd，这表明其在分析活生物系统方面具有很大的潜力。
