Fan Yongzhao, Zhang Linlin, Kong Xiaoyang, Liu Kun, Wu Hao
66551Capital University of Physical Education and Sports, Comprehensive Key Laboratory of Sports Ability Evaluation and Research of the General Administration of Sport of China, Beijing Key Laboratory of Sports Function Assessment and Technical Analysis, Beijing, 100191, China.
Provincial University Key Laboratory of Sport and Health Science, School of Physical Education and Sport Sciences, 12425Fujian Normal University, Fuzhou, China.
Am J Alzheimers Dis Other Demen. 2022 Jan-Dec;37:15333175221082743. doi: 10.1177/15333175221082743.
Previous studies have demonstrated that pre-exercise suppresses anxiety-like behavior, but the effects of different exercise times on vascular dementia induced anxiety-like behavior have not been well investigated.
The present study aims to investigate the underlying neurochemical mechanism of different pre-vascular-dementia exercise times on 5-HT and anxiety-like behavior in rats with vascular dementia.
32 Sprague-Dawley (SD) rats were randomly divided into 4 groups: sham group (S group, n = 8), vascular dementia group (VD group, n = 8), 1-week physical exercise and vascular dementia group (1WVD group, n = 8), and 4 weeks physical exercise and vascular dementia group (4WVD group, n = 8). 1 week and 4 weeks of voluntary wheel running were used as pre-exercise training. The vascular dementia model was established by bilateral common carotid arteries occlusion (BCCAo) for 1 week. But bilateral common carotid arteries were not ligated in the sham group. The level of hippocampal 5-HT was detected with in vivo microdialysis coupled with high-performance liquid chromatography (MD-HPLC). Elevated plus maze (EPM), open field (OF), and light/dark box test were used to test anxiety-like behavior.
Compared with the C group, the hippocampal 5-HT was significantly decreased in the VD group after 1 week of ligated operation. The hippocampal 5-HT levels in 1WVD and 4WVD groups were substantially higher than the level in the VD group. The hippocampal 5-HT level has no significant difference among C, 1WVD, and 4WVD. Behavioral data suggested that the rats in the VD group developed obvious anxiety-like behavior after 1 week of ligation surgery. Still, the rats in 1WVD and 4WVD groups did not show significant anxiety-like behavior.
Both 1 week and 4 weeks of voluntary running wheel exercise can inhibit the anxiety-like behavior in rats with vascular dementia by upregulating 5-HT levels in the hippocampus in the VD model.
先前的研究表明运动前可抑制焦虑样行为，但不同运动时间对血管性痴呆诱导的焦虑样行为的影响尚未得到充分研究。
本研究旨在探讨血管性痴呆运动前不同时间对血管性痴呆大鼠5-羟色胺（5-HT）及焦虑样行为的潜在神经化学机制。
将32只Sprague-Dawley（SD）大鼠随机分为4组：假手术组（S组，n = 8）、血管性痴呆组（VD组，n = 8）、1周体育锻炼与血管性痴呆组（1WVD组，n = 8）和4周体育锻炼与血管性痴呆组（4WVD组，n = 8）。以1周和4周的自愿轮转跑步作为运动前训练。通过双侧颈总动脉闭塞（BCCAo）1周建立血管性痴呆模型。但假手术组未结扎双侧颈总动脉。采用体内微透析结合高效液相色谱法（MD-HPLC）检测海马5-HT水平。采用高架十字迷宫（EPM）、旷场试验（OF）和明暗箱试验检测焦虑样行为。
与假手术组相比，结扎手术1周后VD组海马5-HT显著降低。1WVD组和4WVD组海马5-HT水平显著高于VD组。假手术组、1WVD组和4WVD组海马5-HT水平无显著差异。行为学数据表明，结扎手术1周后VD组大鼠出现明显的焦虑样行为。但1WVD组和4WVD组大鼠未表现出明显的焦虑样行为。
在血管性痴呆模型中，1周和4周的自愿轮转跑步运动均可通过上调海马5-HT水平抑制血管性痴呆大鼠的焦虑样行为。
Zotero 插件
