Demonstration of OKT6 antigen on human thymic dendritic cells in culture.

In situ, two types of dendritic cells (DCs) have been characterized in more detail: the interdigitating DCs of peripheral lymphoid tissues and the epidermal Langerhans cells. In order to characterize human thymic DCs, normal human thymus specimens were obtained from children undergoing cardiovascular surgery. In culture, DCs could be easily differentiated from macrophages and epithelial cells by their long fine processes, irregular nucleus, and dark, membrane-bound granules. By immunoelectron microscopy, using the protein A-gold and the avidin-biotinperoxidase complex techniques, cultured DCs were strongly labeled with anti-Ia and anti-OKT6 monoclonal antibodies. These results demonstrate that the phenotype of thymic DCs is similar to that of epidermal Langerhans cells and suggest that they may belong to the same cellular lineage.