Damjanov I, Mildner B, Knowles B B
Lab Invest. 1986 Nov;55(5):588-92.
A monoclonal antibody recognizing an epitope of the external domain of the human epidermal growth factor (EGF) receptor was used to localize this protein in selected normal human tissues. Two patterns of reactivity were recognized: strong linear or granular cell surface staining, and granular cytoplasmic staining. In one tissue, the endometrium, a change in the reaction pattern associated with changes in hormonal stimulation was observed. In some tissues such as epididymis and skin, the antibody showed surface reactivity with cells considered to represent part of the proliferating cell compartment, whereas in liver, pancreas, and prostate, all cells were reactive with the antibody, though the predominant reactivity was localized in the cytoplasm. The differential distribution of the epidermal growth factor receptor to specific cell types and cellular compartments may signify adaptations that permit growth factor responsiveness in a milieu of available ligand.
一种识别人类表皮生长因子（EGF）受体胞外域表位的单克隆抗体被用于在选定的正常人体组织中定位该蛋白。识别出两种反应模式：强烈的线性或颗粒状细胞表面染色，以及颗粒状胞质染色。在一种组织即子宫内膜中，观察到反应模式的变化与激素刺激的变化相关。在一些组织如附睾和皮肤中，该抗体对被认为代表增殖细胞区室一部分的细胞表现出表面反应性，而在肝脏、胰腺和前列腺中，所有细胞都与该抗体反应，尽管主要反应性定位于细胞质中。表皮生长因子受体在特定细胞类型和细胞区室中的差异分布可能意味着在可利用配体环境中允许生长因子反应性的适应性变化。
