Key Laboratory of Applied Technology on Green-Eco Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Zhejiang 311300, China.
Key Laboratory of Applied Technology on Green-Eco Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Zhejiang 311300, China.
Poult Sci. 2022 May;101(5):101805. doi: 10.1016/j.psj.2022.101805. Epub 2022 Feb 24.
Phosphoglycerate dehydrogenase (PHGDH) is the rate-limiting enzyme in the serine synthesis pathway. However, the regulatory role of PHGDH in muscle development is unclear. We report that the expression of PHGDH increased significantly during proliferation of chicken skeletal muscle satellite cells. Knockdown of PHGDH by an siRNA suppressed myoblast proliferation, whereas overexpression of PHGDH enhanced muscle cell proliferation. Furthermore, PHGDH promoted the expression of Forkhead box protein M1 (FoxM1). Knockdown of FoxM1 by an siRNA attenuated the proliferation of chicken muscle cells, whereas its overexpression significantly promoted proliferation. Additionally, siRNA-PHGDH inhibited pcDNA3.1-FoxM1-induced FoxM1 expression in chicken muscle cells. Moreover, PHGDH inhibition overcame the stimulation by pcDNA3.1-FoxM1 of cell cycle-related gene expression. We propose that PHGDH accelerates chicken muscle cell proliferation by increasing FoxM1 expression.
磷酸甘油酸脱氢酶（PHGDH）是丝氨酸合成途径中的限速酶。然而，PHGDH 在肌肉发育中的调节作用尚不清楚。我们报告说，PHGDH 的表达在鸡骨骼肌卫星细胞的增殖过程中显著增加。通过 siRNA 敲低 PHGDH 抑制成肌细胞增殖，而过表达 PHGDH 则增强肌肉细胞增殖。此外，PHGDH 促进了 Forkhead box 蛋白 M1（FoxM1）的表达。通过 siRNA 敲低 FoxM1 减弱了鸡肌肉细胞的增殖，而过表达则显著促进了增殖。此外，siRNA-PHGDH 抑制了 pcDNA3.1-FoxM1 在鸡肌肉细胞中诱导的 FoxM1 表达。此外，PHGDH 抑制克服了 pcDNA3.1-FoxM1 对细胞周期相关基因表达的刺激。我们提出，PHGDH 通过增加 FoxM1 的表达来加速鸡肌肉细胞的增殖。
