Aix-Marseille Université, CNRS, ISM UMR 7287, Marseille, France; Aix-Marseille Université, CNRS, Centrale Marseille, IRPHE UMR 7342, Marseille, France.
Aix-Marseille Université, CNRS, Centrale Marseille, LMA UMR 7031, Marseille, France.
Ultrasonics. 2022 Aug;124:106714. doi: 10.1016/j.ultras.2022.106714. Epub 2022 Mar 23.
Ultrasound stimulation of living tissues is a promising technique that can be safely applied for regenerative treatments. However, the ultrasound-induced mechanotransduction is still not well understood because of the large number of parameters involved at different scales and their difficult experimental accessibility. In this context, in-vitro studies may help to gain insight into the interaction between ultrasound and cells. Nevertheless, to conduct a reliable analysis of ultrasound effects on cell culture, the monitoring of the acoustic intensity delivered to the cells is of prime interest. Thanks to the development of an innovative custom experimental set-up inspired from ultrasound stimulation of bone regeneration conditions, major disturbing phenomena such as multiple reflections and standing wave formation inside the Petri dish are eliminated. Thus, the level of ultrasound stimulation, especially, in terms of spatial average temporal average intensity (I), delivered to the cells can be monitored. Then, to properly estimate the level of ultrasound stimulation, a finite element model representing the experimental in-vitro configuration is developed. The numerical model manages on capturing the characteristics of the experimentally measured acoustic intensity distribution as illustrated by the experimental and numerical I values of 42.3 and 45.8 mW/cm respectively, i.e. a relative difference of 8%. The numerical model would therefore allow exploring data inaccessible to experimental measurement and parametric studies to be carried out and facilitates the investigation of different virtual experimental configurations.
活体组织的超声刺激是一种很有前途的技术，可安全地应用于再生治疗。然而，由于涉及到大量不同尺度的参数，并且这些参数难以进行实验，因此超声诱导的力学转导仍然没有得到很好的理解。在这种情况下，体外研究可能有助于深入了解超声与细胞之间的相互作用。然而，为了对细胞培养中的超声效应进行可靠的分析，对传递到细胞的声强的监测至关重要。由于从骨再生条件的超声刺激中得到灵感的创新定制实验装置的发展，消除了在培养皿内产生的多重反射和驻波等主要干扰现象。因此，可以监测到传递到细胞的超声刺激的水平，特别是空间平均时间平均强度（I）。然后，为了正确估计超声刺激的水平，开发了一个代表实验体外配置的有限元模型。数值模型成功地捕捉到了实验测量的声强分布的特征，实验和数值的 I 值分别为 42.3 和 45.8 mW/cm，即相对差异为 8%。因此，数值模型可以探索实验测量无法获取的数据，并进行参数研究，方便研究不同的虚拟实验配置。
