人乳头瘤病毒和 Epstein-Barr 病毒感染与泰国东北部扁桃体癌的相关性研究。

Association of Human Papillomavirus and Epstein-Barr Virus Infection with Tonsil Cancer in Northeastern Thailand.

机构信息

Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.

HPV & EBV and Carcinogenesis Research Group, Khon Kaen University, Khon Kaen, Thailand.

出版信息

Asian Pac J Cancer Prev. 2022 Mar 1;23(3):781-787. doi: 10.31557/APJCP.2022.23.3.781.

DOI:10.31557/APJCP.2022.23.3.781

PMID:35345347

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9360956/

Abstract

BACKGROUND

Human papillomavirus (HPV) and Epstein-Barr virus (EBV) are associated with head and neck cancer, including tonsil cancer (TC) in the oropharyngeal area. Increasing incidence of HPV and EBV infection in different cancer tissues of oropharynx in both epithelial and lymphoid tissues, have been reported. However, little is known about association of these tumor viruses with TC in the Thai population. Here, we investigated the prevalence of HPV and EBV infection in different histology of TC and their association with TC from Thai patients.

METHODS

Eighty-three exfoliated tonsil cells from non-cancer controls (NCC) and 65 formalin-fixed paraffin-embedded TC tissues (TC) that were histologically classified as tonsillar squamous-cell carcinoma (TSCC) or diffuse large B-cell lymphoma (DLBCL) were studied. Prevalence of HPV and EBV infection was determined by real-time PCR. HPV genotyping was performed by reverse line blot hybridization and HPV genome status was investigated by multiplex qPCR. Localization of EBV infection was determined by EBER in situ hybridization.

RESULTS

Infection of HPV and EBV in TC cases was 16.9% and 30.8%, whereas in exfoliated tonsil cells was 1.2% and 66.3% respectively. HPV infection was significantly higher in TSCC (30.6%) than DLBCL samples (13.8%). HPV58 was commonly detected and presented as an integrated form in TSCC, whereas only episomal form was found in DLBCL. EBV infection was significantly higher in DLBCL (44.8%) than TSCC samples (19.4%), and detected in both lower than among exfoliated tonsil cell samples (66.3%). By EBER in situ hybridization in TSCC, EBV infection localized both in epithelial cells and infiltrating lymphocytes. The co-occurrence of HPV and EBV infection was 11.11% and 13.79% of TSCC and DLBCL, respectively, was associated with well-differentiated TSCC.

CONCLUSION

HPV and EBV infection was significantly involved in a specific TC tissue, and associated with a good clinical outcome in TSCC.

摘要

背景

人乳头瘤病毒（HPV）和 Epstein-Barr 病毒（EBV）与头颈部癌症有关，包括口咽区域的扁桃体癌（TC）。已经报道了在口咽不同上皮和淋巴组织的癌症组织中 HPV 和 EBV 感染的发病率增加。然而，对于这些肿瘤病毒与泰国人群 TC 的关系知之甚少。在这里，我们研究了 HPV 和 EBV 在 TC 的不同组织学中的感染率及其与泰国患者 TC 的关系。

方法

对 83 例非癌症对照（NCC）脱落的扁桃体细胞和 65 例经组织学分类为扁桃体鳞状细胞癌（TSCC）或弥漫性大 B 细胞淋巴瘤（DLBCL）的 TC 组织进行了研究。通过实时 PCR 确定 HPV 和 EBV 感染的流行率。通过反向线杂交进行 HPV 基因分型，并通过多重 qPCR 研究 HPV 基因组状态。通过 EBER 原位杂交确定 EBV 感染的定位。

结果

TC 病例中 HPV 和 EBV 的感染率分别为 16.9%和 30.8%，而在脱落的扁桃体细胞中分别为 1.2%和 66.3%。HPV 感染在 TSCC（30.6%）中明显高于 DLBCL 样本（13.8%）。HPV58 常见且在 TSCC 中呈整合形式，而在 DLBCL 中仅发现游离形式。DLBCL 中的 EBV 感染明显高于 TSCC 样本（44.8%），而低于脱落的扁桃体细胞样本（66.3%）。在 TSCC 中，通过 EBER 原位杂交检测到 EBV 感染定位于上皮细胞和浸润淋巴细胞。HPV 和 EBV 感染的共发生在 TSCC 和 DLBCL 中分别为 11.11%和 13.79%，与分化良好的 TSCC 相关。