Sargent E V, Bradley M O
Mutat Res. 1986 Nov;175(3):133-7. doi: 10.1016/0165-7992(86)90111-9.
m-Nitrobenzaldehyde (MNB) was evaluated for mutagenic activity using the Ames microbial mutagenicity test and for its ability to induce DNA single-strand breaks in rat hepatocytes as measured by alkaline elution. MNB was tested in S. typhimurium strains TA1535, TA1537, TA1538, TA98, and TA100, both with and without pretreatment with liver microsomes (S9) isolated from rats pretreated with Aroclor 1254. MNB produced 2-fold or greater increases in revertants in TA1538, both with and without S9, and in TA100 with S9 only. A 2-fold increase in revertants was seen in TA98, but only at the highest dose tested which did not produce inhibition of background growth. MNB caused a greater than 3-fold increase in elution slope, with DNA alkaline elution assay, but only at highly cytotoxic doses and, therefore, is not considered genotoxic in this system. It is concluded that MNB possesses weak genotoxic activity.
使用艾姆斯微生物致突变性试验评估间硝基苯甲醛（MNB）的致突变活性，并通过碱性洗脱法测定其诱导大鼠肝细胞DNA单链断裂的能力。MNB在鼠伤寒沙门氏菌菌株TA1535、TA1537、TA1538、TA98和TA100中进行了测试，测试时分别添加和不添加从用多氯联苯1254预处理的大鼠中分离的肝微粒体（S9）。无论有无S9，MNB均使TA1538中的回复突变体增加2倍或更多，仅在有S9时使TA100中的回复突变体增加。在TA98中，仅在测试的最高剂量下观察到回复突变体增加2倍，该剂量未产生对背景生长的抑制。通过DNA碱性洗脱试验，MNB使洗脱斜率增加超过3倍，但仅在高细胞毒性剂量下出现，因此在该系统中不被认为具有遗传毒性。结论是MNB具有弱遗传毒性活性。
