Mu Yinyu, Xie Fuyi, Yang Dongdong, Xu Guodong
Department of Clinical Laboratory, Ningbo Medical Centre, Lihuili Hospital, Ningbo University, Ningbo, Zhejiang, 315040, People's Republic of China.
Department of Cardiothoracic Surgery, Ningbo Medical Centre, Lihuili Hospital, Ningbo University, Ningbo, Zhejiang, 315040, People's Republic of China.
Cancer Manag Res. 2022 Mar 22;14:1229-1236. doi: 10.2147/CMAR.S346616. eCollection 2022.
Circular RNA (circRNA) serves an important role in tumour genesis and development. But, little is known about its role in lung adenocarcinoma (LA). This study aimed to investigate circRNA6783 expression in peripheral whole blood (PWB) of LA and controls and explore its effect on proliferation and apoptosis in human lung adenocarcinoma cells (LAC).
The levels of circRNA6783 in LA cell lines and peripheral whole blood (PWB) of 40 patients and 30 controls were detected by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR). In order to explore the effect of circRNA6783 on LA behavior, we overexpressed circRNA6783 in NCI-H1975 cells. The impact on the proliferation of tumor cells was then examined by Cell Counting Kit 8 (CCK8) assay, and the effects on apoptosis in the cell line were detected using flow cytometry.
The expression levels of circRNA6783 were significantly higher in LA cell lines and PWB (P < 0.05). The diagnostic value of the area under the receiver operating characteristic curve (AUC) was 0.830, with a sensitivity of 60% and specificity of 96.7%. In addition, functional experiments showed that overexpression of circRNA6783 restrained cell proliferation, significantly increased spontaneous apoptosis.
CircRNA6783 was upregulated in LA PWB. In vitro assessment demonstrated that circRNA6783 could act as a potential biomarker for LA diagnosis.
环状RNA（circRNA）在肿瘤发生发展中起重要作用。但是，其在肺腺癌（LA）中的作用鲜为人知。本研究旨在调查circRNA6783在LA患者和对照组外周全血（PWB）中的表达，并探讨其对人肺腺癌细胞（LAC）增殖和凋亡的影响。
通过实时定量逆转录-聚合酶链反应（RT-PCR）检测40例患者和30例对照的LA细胞系及外周全血（PWB）中circRNA6783的水平。为了探讨circRNA6783对LA行为的影响，我们在NCI-H1975细胞中过表达circRNA6783。然后通过细胞计数试剂盒8（CCK8）检测对肿瘤细胞增殖的影响，并使用流式细胞术检测对细胞系凋亡的影响。
circRNA6783在LA细胞系和PWB中的表达水平显著更高（P < 0.05）。受试者工作特征曲线（AUC）下面积的诊断价值为0.830，敏感性为60%，特异性为96.7%。此外，功能实验表明，circRNA6783的过表达抑制细胞增殖，显著增加自发凋亡。
circRNA6783在LA的PWB中上调。体外评估表明，circRNA6783可作为LA诊断的潜在生物标志物。
