Athanasiou K, Arzimanoglou I I
Mutat Res. 1986 Nov;175(3):165-9. doi: 10.1016/0165-7992(86)90117-x.
The mutagenic and clastogenic effects of the antineoplastic agents homo-aza-steroidal ester (ASE) and chlorambucil (CBC) were tested for their ability to induce mutations in the Salmonella/microsome system and SCE in CHO cells in culture. ASE was found to be positive in strains TA1535 and TA100 and in the newer strain TA102 with and without metabolic activation, while CBC caused histidine reversion in strain TA102 after the addition of mammalian liver microsomal extract (S9). In addition, both agents were found to be strongly positive for SCE induction. The mutagenic and clastogenic actions of both agents were of a dose-response type.
对抗肿瘤药物高氮杂甾体酯（ASE）和苯丁酸氮芥（CBC）的诱变和致断裂作用进行了测试，以检验它们在沙门氏菌/微粒体系统中诱导突变以及在培养的CHO细胞中诱导姐妹染色单体交换（SCE）的能力。结果发现，ASE在TA1535和TA100菌株以及较新的TA102菌株中，无论有无代谢活化均呈阳性；而CBC在添加哺乳动物肝微粒体提取物（S9）后，可使TA102菌株发生组氨酸回复突变。此外，还发现这两种药物对SCE诱导均呈强阳性。两种药物的诱变和致断裂作用均呈剂量反应型。
