通过二维样本分级分离结合串联质谱法获得的人类肝脏样本深度蛋白质组数据集。
Deep proteomic dataset of human liver samples obtained by two-dimensional sample fractionation coupled with tandem mass spectrometry.
作者信息
Vavilov Nikita E, Ilgisonis Ekaterina V, Lisitsa Andrey V, Ponomarenko Elena A, Farafonova Tatiana E, Tikhonova Olga V, Zgoda Victor G, Archakov Alexander I
机构信息
Institute of Biomedical Chemistry, Pogodinskaya street 10, bld. 7, Moscow, Russia.
出版信息
Data Brief. 2022 Mar 12;42:108055. doi: 10.1016/j.dib.2022.108055. eCollection 2022 Jun.
The data was acquired from 3 normal human liver tissues by LC-MS methods. The tissue liver samples from male subjects post mortem were obtained from ILSBio LLC (https://bioivt.com/). Liver tissue was frozen in liquid nitrogen, transported and shipped on dry ice. The proteins were extracted and purified followed up by trypsin hydrolysis. The peptide mixture was aliquoted and analyzed by different LC-MS approaches: one-dimensional shotgun LC-MS, two-dimensional LC-MS, two-dimensional SRM SIS (Selected Reaction Monitoring with Stable Isotope-labeled peptide Standards). The Shotgun assay resulted in a qualitative in-depth human liver proteome, and a semi-quantitative iBAQ (intensity-based absolute quantification) value was calculated to show the relative protein content of the sample. Absolute quantitative concentrations of proteins encoded by human chromosome 18 using SRM SIS were obtained.
数据通过液相色谱-质谱联用（LC-MS）方法从3份正常人类肝脏组织中获取。男性受试者死后的肝脏组织样本购自ILSBio LLC（https://bioivt.com/）。肝脏组织在液氮中冷冻，用干冰运输和运送。蛋白质经提取、纯化后进行胰蛋白酶水解。肽混合物被等分，并通过不同的LC-MS方法进行分析：一维鸟枪法LC-MS、二维LC-MS、二维选择反应监测-稳定同位素标记肽标准品（SRM SIS）。鸟枪法分析得出了定性的深度人类肝脏蛋白质组，并计算了基于强度的绝对定量（iBAQ）半定量值以显示样品中蛋白质的相对含量。使用SRM SIS获得了人类18号染色体编码蛋白质的绝对定量浓度。
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