Department of Maternal and Child Health, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, 250012, Shandong, China.
Department of Thoracic Surgery, Heze Municipal Hospital, Heze, 274031, Shandong, China.
World J Surg Oncol. 2022 Mar 28;20(1):96. doi: 10.1186/s12957-022-02566-6.
Lung cancer is the leading cause of cancer-related mortality. STEAP1 and STEAP2 are overexpressed in various cancers. The purpose of this study was to evaluate the expression and prognostic value of STEAP1 and STEAP2 in patients with lung cancer.
The mRNA expression and protein expression of STEAP1 and STEAP2 and their prognostic characteristics were examined using Oncomine, GEPIA, and Kaplan-Meier (KM) plotters. The correlation analysis of STEAP1 and STEAP2 gene and protein levels was conducted using GeneMANIA and STRING. KEGG pathway analysis was used to explore the related signal pathways of STEAP 1 and STEAP2. Immunohistochemical methods were used to compare the expression of STEAP2 in normal lung and non-small cell lung cancer (NSCLC) tissues. Real-time quantitative polymerase chain reaction, western blotting, and immunocytochemistry were used to evaluate the expression of STEAP1 and STEAP2 in three lung cancer cell lines and normal lung epithelial cell lines.
Analysis of the Oncomine database and GEPIA showed that STEAP1 was upregulated and STEAP2 was downregulated in lung cancer tissue, and both expressions were related to the clinical stage of lung cancer. Immunohistochemical analysis showed that STEAP1 protein expression was significantly upregulated in lung cancer compared to that in adjacent tissues. The expression of STEAP1 was positively correlated with the migration and invasion abilities of lung cancer cells. Compared with paracancer tissues, the expression of STEAP2 protein in lung cancer was significantly downregulated and was correlated with the histological grade of squamous cell carcinoma, pathological classification of adenocarcinoma, tumor, lymph node, and metastasis clinical stage, and lymph node metastasis. The expression of STEAP2 was negatively correlated with the migration and invasion abilities of lung cancer cells. The KM curve showed that the downregulation of STEAP1 expression and upregulation of STEAP2 expression were related to a good lung cancer prognosis.
STEAP1 and STEAP2 are expected to be potential diagnostic and prognostic markers for lung cancer, which may provide more accurate prognostic indicators for lung cancer.
肺癌是癌症相关死亡的主要原因。STEAP1 和 STEAP2 在各种癌症中过度表达。本研究旨在评估 STEAP1 和 STEAP2 在肺癌患者中的表达及其预后价值。
使用 Oncomine、GEPIA 和 Kaplan-Meier（KM）绘图器检查 STEAP1 和 STEAP2 的 mRNA 表达和蛋白表达及其预后特征。使用 GeneMANIA 和 STRING 进行 STEAP1 和 STEAP2 基因和蛋白水平的相关性分析。KEGG 途径分析用于探索 STEAP1 和 STEAP2 的相关信号通路。免疫组织化学方法用于比较正常肺组织和非小细胞肺癌（NSCLC）组织中 STEAP2 的表达。实时定量聚合酶链反应、蛋白质印迹和免疫细胞化学用于评估三种肺癌细胞系和正常肺上皮细胞系中 STEAP1 和 STEAP2 的表达。
Oncomine 数据库和 GEPIA 分析表明，STEAP1 在肺癌组织中上调，STEAP2 下调，两者的表达均与肺癌的临床分期有关。免疫组织化学分析显示，与相邻组织相比，肺癌组织中 STEAP1 蛋白表达明显上调。STEAP1 的表达与肺癌细胞的迁移和侵袭能力呈正相关。与癌旁组织相比，肺癌组织中 STEAP2 蛋白表达明显下调，与鳞状细胞癌的组织学分级、腺癌的病理分类、肿瘤、淋巴结和转移的临床分期以及淋巴结转移相关。STEAP2 的表达与肺癌细胞的迁移和侵袭能力呈负相关。KM 曲线表明，STEAP1 表达下调和 STEAP2 表达上调与肺癌的良好预后相关。
STEAP1 和 STEAP2 有望成为肺癌的潜在诊断和预后标志物，可为肺癌提供更准确的预后指标。
