Sherbrooke Research and Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, Québec, Canada J1M 0C8; Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Sherbrooke, QC, Canada J1K 2R1.
Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Sherbrooke, QC, Canada J1K 2R1.
J Dairy Sci. 2022 May;105(5):4498-4507. doi: 10.3168/jds.2021-21381. Epub 2022 Mar 26.
The use of teat dips is one of the most effective strategies to control mastitis by preventing new intramammary infections. Reducing bacterial load on teat skin helps control the spread of pathogens and spoilage and improves the quality of milk. The objective of this study was to evaluate the reduction of bacterial populations through the application of bacteriocin-based teat formulas. Teats of 12 Holstein cows received 2 different concentrations of bactofencin A, nisin, and reuterin alone or in combination, as well as iodine (positive control) and saline (negative control). Teat swabs were collected before and after application of teat formulas and analyzed for staphylococci, streptococci, and total bacteria counts. There were no differences for staphylococci, streptococci, and total bacterial counts for samples collected before application throughout the entire experiment. Reuterin-low and reuterin-high treatments reduced total bacterial count by 0.47 and 0.36 logs, respectively, whereas bactofencin A had no effect on any tested bacterial groups. Nisin-low treatment reduced staphylococci, streptococci, and total bacterial counts by 0.47, 0.30 and 0.50 logs, respectively. Nisin-high treatment resulted in 0.50, 0.50, and 0.47 log reduction for staphylococci, streptococci, and total bacterial counts. The bacteriocin consortium showed the highest reduction rates with 0.91, 0.54, and 0.90 log reductions obtained for staphylococci, streptococci, and total bacteria counts, respectively, for the low-concentration consortium. Similarly, the high-concentration consortium showed reduction rates with 0.95, 0.60, and 0.82 log reductions obtained for staphylococci, streptococci, and total bacteria counts, respectively. Thus, nisin and the bacteriocin consortium showed the most promise as a teat disinfectant by reducing staphylococci, streptococci, and total bacteria counts.
药浴是控制乳腺炎的最有效策略之一，通过防止新的乳房内感染来实现。减少乳头皮肤上的细菌负荷有助于控制病原体和腐败物的传播，并提高牛奶质量。本研究的目的是评估通过应用细菌素为基础的乳头配方来减少细菌种群。12 头荷斯坦奶牛的乳头接受了单独或组合使用 bactofencin A、乳链菌肽和雷普霉素的 2 种不同浓度，以及碘（阳性对照）和生理盐水（阴性对照）的处理。在应用乳头配方前后采集乳头拭子，并对葡萄球菌、链球菌和总细菌计数进行分析。在整个实验过程中，应用前采集的样本中葡萄球菌、链球菌和总细菌计数没有差异。雷普霉素低和雷普霉素高处理分别减少了 0.47 和 0.36 个对数的总细菌计数，而 bactofencin A 对任何测试的细菌群都没有影响。乳链菌肽低处理分别减少了 0.47、0.30 和 0.50 个对数的葡萄球菌、链球菌和总细菌计数。乳链菌肽高处理导致葡萄球菌、链球菌和总细菌计数分别减少 0.50、0.50 和 0.47 个对数。细菌素联合体表现出最高的减少率，低浓度联合体分别获得了葡萄球菌、链球菌和总细菌计数 0.91、0.54 和 0.90 个对数的减少率。同样，高浓度联合体分别获得了葡萄球菌、链球菌和总细菌计数 0.95、0.60 和 0.82 个对数的减少率。因此，乳链菌肽和细菌素联合体通过减少葡萄球菌、链球菌和总细菌计数，显示出作为乳头消毒剂的最大潜力。
