School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, China.
Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai, 200444, China.
Mikrochim Acta. 2022 Mar 26;189(4):155. doi: 10.1007/s00604-022-05269-7.
There is an urgent need for a flexible and simple programmed cell death ligand 1 (PD-L1) dynamic measurement method enabling real-time monitoring of cancer progression and assessment of immunotherapy efficacy. In the current study, we show facile in situ synthesis of vertical alignment two-dimensional molybdenum disulfide (2D MoS) layers on graphene-oxide-modified ITO (MoSGO-ITO) using a hydrothermal approach and demonstrate the importance of the alignment of 2D in achieving high-probe capturing, enhanced electrochemical properties and target selectivity during sensing. After modification of designed PD-L1 binding peptides on the MoSGO-ITO, a sensitive PD-L1 electrochemical sensor was designed using vertical alignment MoS to capture more probes for PD-L1 recognition and excellent in plane electron transport to accelerate electrochemical signals. The fabricated electrochemical sensor could sensitively determine PD-L1 in a wide linear range of 25-500 ng/mL and exhibit desirable accuracy and reliability in clinical samples application. This simple and sensitive method is likely to investigate further research into the exploration of the perpendicular alignment of 2D surfaces for diverse applications.
目前迫切需要一种灵活且简单的程序性细胞死亡配体 1（PD-L1）动态测量方法，以实现对癌症进展的实时监测和免疫疗法效果的评估。在本研究中，我们展示了一种简便的原位合成方法，即在氧化石墨烯修饰的 ITO（MoSGO-ITO）上使用水热法合成垂直排列的二维二硫化钼（2D MoS）层，并证明了在实现高探针捕获、增强电化学性能和目标选择性方面，2D 的排列的重要性。在将设计的 PD-L1 结合肽修饰到 MoSGO-ITO 上之后，使用垂直排列的 MoS 设计了一种灵敏的 PD-L1 电化学传感器，以捕获更多的探针用于 PD-L1 识别，并具有优异的平面电子传输以加速电化学信号。所制备的电化学传感器能够在 25-500ng/mL 的宽线性范围内灵敏地测定 PD-L1，并在临床样品应用中表现出良好的准确性和可靠性。这种简单灵敏的方法有望进一步研究用于各种应用的二维表面的垂直排列。
