College of Chemistry and Materials Science, Inner Mongolia Key Laboratory of Carbon Nanomaterials, Nano Innovation Institute (NII), Inner Mongolia Minzu University, Tongliao, 028000, People's Republic of China.
Mikrochim Acta. 2022 Mar 26;189(4):156. doi: 10.1007/s00604-022-05258-w.
A layer-by-layer (LbL) assembly strategy was developed to prepare multilayered double hydroxide/polyoxometalate shell-coated magnetic nanoparticles. The introduction of functional shells not only offered abundant affinity sites of metal oxide and metal ions but also increased the surface area for the contact with targets. By combining the enrichment strategies of immobilized metal ion affinity chromatography and metal oxide affinity chromatography, the nanomaterial can capture phosphopeptides via a synergistic effect. The method presented a low detection limit of 0.1 fmol in combination with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) analysis. The nanomaterial showed satisfactory selectivity (1:1:5000 M ratio of α-/β-casein/bovine serum albumin), good recovery (92.07%), high adsorption capacity (117.6 mg g), and ten times reusability for capturing phosphopeptides.
一种层层（LbL）组装策略被开发出来，用于制备多层氢氧化物/多金属氧酸盐壳层包覆的磁性纳米粒子。功能壳层的引入不仅提供了丰富的金属氧化物和金属离子的亲和位点，而且增加了与目标物接触的表面积。通过结合固定化金属离子亲和色谱和金属氧化物亲和色谱的富集策略，该纳米材料可以通过协同作用捕获磷酸肽。该方法与基质辅助激光解吸/电离飞行时间（MALDI-TOF）分析相结合，检测限低至 0.1 fmol。该纳米材料具有令人满意的选择性（α-/β-酪蛋白/牛血清白蛋白的比例为 1:1:5000）、良好的回收率（92.07%）、高的吸附容量（117.6 mg g）和 10 倍的磷酸肽捕获重复使用性。
