Jiang Dandan, Duan Limei, Jia Qiong, Liu Jinghai
Inner Mongolia Key Laboratory of Carbon Nanomaterials, Nano Innovation Institute (NII), College of Chemistry and Materials Science, Inner Mongolia University for Nationalities, Tongliao, 028000, PR China.
College of Chemistry, Jilin University, Changchun, 130012, PR China.
Anal Chim Acta. 2020 Nov 1;1136:25-33. doi: 10.1016/j.aca.2020.07.057. Epub 2020 Aug 20.
Efficient enrichment and identification of phosphopeptides are of great significance in biological applications. Glycocyamine functionalized magnetic layered double hydroxides (FeO@LDH@NH-GAA) was fabricated through an easy process. The magnetic composite possessed high surface area, good biocompatibility, and fast magnetic response. FeO@LDH@NH-GAA, combining not only metal ions (Cu and Ga) in LDH but also functional guanidyl groups in glycocyamine, offered multiple affinity sites for phosphopeptides enrichment. With these favorable characters, it exhibited high selectivity (β-casein: bovine serum albumin = 1:5000), low detection limit (0.1 fmol), satisfactory enrichment recovery (94.5%), high adsorption capacity (82.4 mg g), and good repetitiveness. Moreover, the efficient enrichment of phosphopeptides by FeO@LDH@NH-GAA from nonfat milk, human saliva, serum, and A549 cell lysates further confirmed its great potential for trace biological detection and proteomic analysis.
高效富集和鉴定磷酸化肽段在生物应用中具有重要意义。通过简便的过程制备了胍基乙胺功能化的磁性层状双氢氧化物（FeO@LDH@NH-GAA）。该磁性复合材料具有高比表面积、良好的生物相容性和快速的磁响应。FeO@LDH@NH-GAA不仅结合了层状双氢氧化物中的金属离子（铜和镓），还结合了胍基乙胺中的功能性胍基，为磷酸化肽段的富集提供了多个亲和位点。凭借这些优良特性，它表现出高选择性（β-酪蛋白：牛血清白蛋白 = 1:5000）、低检测限（0.1 fmol）、令人满意的富集回收率（94.5%）、高吸附容量（82.4 mg g）和良好的重复性。此外，FeO@LDH@NH-GAA从脱脂牛奶、人唾液、血清和A549细胞裂解物中高效富集磷酸化肽段，进一步证实了其在痕量生物检测和蛋白质组学分析方面的巨大潜力。
