College of Life Science, Yangtze University, 266 Jingmi Road, Jingzhou, 434025, Hubei, China.
State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, China.
Mikrochim Acta. 2022 Mar 26;189(4):158. doi: 10.1007/s00604-022-05284-8.
A rapid and convenient fluorescence glyphosate (GLYP) biosensor was developed based on DNA-templated copper nanoparticles (DNA-CuNPs). In the absence of GLYP, the DNA-CuNPs were formed through the reduction of Cu by vitamin C (Vc). The DNA-CuNPs emitted intense fluorescence at 615 nm when being excited at 340 nm. In the presence of GLYP, GLYP can strongly chelate with Cu by the phosphate and carboxyl groups to decrease the amount of free Cu. Due to the lack of free Cu, DNA-CuNPs cannot be formed, which caused the fluorescence to decrease. The whole detection process of this proposed GLYP biosensor can be completed within 14 min. Titration experiments showed that this biosensor had a linear relationship for GLYP in the range 1 to 18 µM with a limit of detection (LOD) of 0.47 µM. This biosensor showed obvious selectivity among other pesticides, even between GLYP and organophosphorus pesticides. This biosensor performed well for GLYP detection in real samples with recoveries of 88.0-104.0%.
基于 DNA 模板铜纳米粒子（DNA-CuNPs），开发了一种快速简便的荧光草甘膦（GLYP）生物传感器。在没有 GLYP 的情况下，Cu 被维生素 C（Vc）还原形成 DNA-CuNPs。当在 340nm 激发时，DNA-CuNPs 在 615nm 处发射出强烈的荧光。在存在 GLYP 的情况下，GLYP 可以通过磷酸根和羧基与 Cu 强烈螯合，从而减少游离 Cu 的量。由于缺乏游离的 Cu，DNA-CuNPs 无法形成，导致荧光减弱。该提出的 GLYP 生物传感器的整个检测过程可以在 14 分钟内完成。滴定实验表明，该生物传感器在 1 至 18µM 的范围内对 GLYP 具有线性关系，检测限（LOD）为 0.47µM。该生物传感器在其他农药之间甚至在 GLYP 和有机磷农药之间表现出明显的选择性。该生物传感器在实际样品中的 GLYP 检测中表现良好，回收率为 88.0-104.0%。
