Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation, Hunan Provincial Engineering Research Center for Food Processing of Aquatic Biotic Resources, School of Chemistry and Food Engineering, Changsha University of Science and Technology, Changsha, 410114, PR China.
Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation, Hunan Provincial Engineering Research Center for Food Processing of Aquatic Biotic Resources, School of Chemistry and Food Engineering, Changsha University of Science and Technology, Changsha, 410114, PR China.
Biosens Bioelectron. 2019 Jul 15;137:96-109. doi: 10.1016/j.bios.2019.05.014. Epub 2019 May 7.
During the last decades, by virtue of their unique physicochemical properties and potential application in microelectronics, biosensing and biomedicine, metal nanomaterials (MNs) have attracted great research interest and been highly developed. Deoxyribonucleic acid (DNA) is a particularly interesting ligand for templating bottom-up nanopreparation, by virtue of its excellent properties including nanosized geometry structure, programmable and artificial synthesis, DNA-metal ion interaction and powerful molecular recognition. DNA-templated copper nanoparticles (DNA-CuNPs) has been developed in recent years. Because of its advantages including simple and rapid preparation, high efficiency, MegaStokes shifting and low biological toxicity, DNA-CuNPs has been highly exploited for biochemical sensing from 2010, especially as a label-free detection manner, holding advantages in multiple analytical technologies including fluorescence, electrochemistry, surface plasmon resonance, inductively coupled plasma mass spectrometry and surface enhanced Raman spectroscopy. This review comprehensively tracks the preparation of DNA-CuNPs and its application in biosensing, and highlights the potential development and challenges regarding this field, aiming to promote the advance of this fertile research area.
在过去几十年中，由于其独特的物理化学性质以及在微电子、生物传感和生物医学方面的潜在应用，金属纳米材料 (MNs) 引起了极大的研究兴趣并得到了高度发展。脱氧核糖核酸 (DNA) 是一种特别有趣的配体，可用于模板化自下而上的纳米制备，这得益于其包括纳米级几何结构、可编程和人工合成、DNA-金属离子相互作用和强大的分子识别在内的优异性质。近年来已经开发出 DNA 模板铜纳米颗粒 (DNA-CuNPs)。由于其具有制备简单快速、效率高、Stokes 位移大、生物毒性低等优点，自 2010 年以来，DNA-CuNPs 已被高度用于生化传感，特别是作为无标记检测方式，在包括荧光、电化学、表面等离子体共振、电感耦合等离子体质谱和表面增强拉曼光谱在内的多种分析技术中具有优势。本综述全面跟踪了 DNA-CuNPs 的制备及其在生物传感中的应用，并重点介绍了该领域的潜在发展和挑战，旨在促进这一富有成效的研究领域的发展。
