Tonouchi N, Shoun H, Uozumi T, Beppu T
Nucleic Acids Res. 1986 Oct 10;14(19):7557-68. doi: 10.1093/nar/14.19.7557.
The aspartate protease of Mucor pusillus (Mucor pusillus rennin; MPR) is a milk-clotting enzyme used in the cheese industry. The partial amino acid sequence of MPR was determined and oligonucleotide probes were synthesized for cloning of the MPR gene. A clone giving positive hybridization with the probes was selected from the cosmid library. Sequencing of the cloned DNA revealed an open reading frame of 1281 bp without introns which encodes 361 amino acids for the expected MPR with an NH2-terminal extension of 66 amino acids. MPR seems to be synthesized as a prepro enzyme.
微小毛霉的天冬氨酸蛋白酶（微小毛霉凝乳酶；MPR）是一种用于奶酪工业的凝乳酶。测定了MPR的部分氨基酸序列，并合成了寡核苷酸探针用于克隆MPR基因。从黏粒文库中筛选出与探针呈阳性杂交的克隆。对克隆的DNA进行测序，发现一个1281 bp的开放阅读框，无内含子，编码预期的含66个氨基酸N端延伸的361个氨基酸的MPR。MPR似乎以前体酶的形式合成。
