Division of Life Sciences, Korea Polar Research Institute, 26 Songdomirae-ro, Yeonsu-gu, Incheon 21990, Republic of Korea.
School of Earth and Environmental Sciences and Research Institute of Oceanography, Seoul National University, Seoul 08826, Republic of Korea.
Int J Syst Evol Microbiol. 2022 Mar;72(3). doi: 10.1099/ijsem.0.005290.
Gram-stain-negative, strictly aerobic, red-pink-coloured, rod-shaped and non-motile bacterial strains PAMC 29290, PAMC 29294 and PAMC 29296 were isolated from marine surface sediment sampled in the East Siberian Sea and strains PAMC 26553 and PAMC 26554 were obtained from an Antarctic lichen. Strains PAMC 29290, PAMC 29294 and PAMC 29296 were closely related to (98.8 % 16S rRNA gene similarity), (97.3 %) and (96.9 %), and PAMC 26553 and PAMC 26554 showed high similarity to (97.0 %), (96.1 %) and (95.9 %). Genomic relatedness analyses showed that strains PAMC 29290, PAMC 29294 and PAMC 29296 could be distinguished from by average nucleotide identity (ANI; 93.1-93.2 %) and digital DNA-DNA hybridization (dDDH; 50.3-51.0 %) values. Strains PAMC 26553 and PAMC 26554 could be clearly distinguished from with ANI values <79.8 % and dDDH values <23.3 %. The major fatty acids of strains PAMC 29290, PAMC 29294 and PAMC 29296 were C iso (21.0-26.0 %), summed feature 3 (C ω7 and/or C ω6; 17.4-18.2 %), C anteiso (12.7-19.1 %) and summed feature 4 (C iso I and/or anteiso B; 8.6-16.1 %) and those of strains PAMC 26553 and PAMC 26554 were summed feature 3 (C ω7 and/or C ω6; 20.7-22.2 %), C anteiso (17.5-19.7 %) and summed feature 4 (C iso I and/or anteiso B; 15.5-18.1 %). The major respiratory quinone was MK-7. The genomic DNA G+C contents were 60.6-60.8 mol%. The polar lipids of PAMC 29294 were found to consist of phosphatidylethanolamine, four unidentified aminolipids, an unidentified aminophospholipid and five unidentified lipids; those of PAMC 26554 were phosphatidylethanolamine, three unidentified aminolipids, four unidentified aminophospholipid and two unidentified lipids. The distinct phylogenetic position and some physiological characteristics distinguished the novel strains from closely related type strains in the genus . Thus, two novel species are proposed, with the names sp. nov. (type strain, PAMC 29294=KCTC 82466=JCM 34574) and sp. nov. (type strain, PAMC 26554=KCTC 82464=JCM 34572), respectively.
革兰氏染色阴性、严格需氧、粉红色的杆状、非运动的细菌菌株 PAMC 29290、PAMC 29294 和 PAMC 29296 从东西伯利亚海域的海洋表层沉积物中分离得到，而菌株 PAMC 26553 和 PAMC 26554 则从南极地衣中获得。菌株 PAMC 29290、PAMC 29294 和 PAMC 29296 与 （98.8% 的 16S rRNA 基因相似度）、 （97.3%）和 （96.9%）密切相关，而 PAMC 26553 和 PAMC 26554 与 （97.0%）、 （96.1%）和 （95.9%）具有高度相似性。基因组相关性分析表明，菌株 PAMC 29290、PAMC 29294 和 PAMC 29296 可以通过平均核苷酸同一性（ANI；93.1-93.2%）和数字 DNA-DNA 杂交（dDDH；50.3-51.0%）值与 区分开来。菌株 PAMC 26553 和 PAMC 26554 可以通过 ANI 值<79.8%和 dDDH 值<23.3%与 清楚地区分。菌株 PAMC 29290、PAMC 29294 和 PAMC 29296 的主要脂肪酸为 C iso（21.0-26.0%）、summed feature 3（C ω7 和/或 C ω6；17.4-18.2%）、C anteiso（12.7-19.1%）和 summed feature 4（C iso I 和/或 anteiso B；8.6-16.1%），而菌株 PAMC 26553 和 PAMC 26554 的主要脂肪酸为 summed feature 3（C ω7 和/或 C ω6；20.7-22.2%）、C anteiso（17.5-19.7%）和 summed feature 4（C iso I 和/或 anteiso B；15.5-18.1%）。主要的呼吸醌为 MK-7。基因组 DNA G+C 含量为 60.6-60.8 mol%。PAMC 29294 的极性脂由磷脂酰乙醇胺、四种未鉴定的胺脂、一种未鉴定的胺磷脂和五种未鉴定的脂组成；PAMC 26554 的极性脂由磷脂酰乙醇胺、三种未鉴定的胺脂、四种未鉴定的胺磷脂和两种未鉴定的脂组成。新菌株与属内密切相关的模式菌株在独特的系统发育位置和一些生理特性上有所不同。因此，提出了两个新种，分别命名为 sp. nov.（模式菌株，PAMC 29294=KCTC 82466=JCM 34574）和 sp. nov.（模式菌株，PAMC 26554=KCTC 82464=JCM 34572）。
