Division of Polar Life Sciences, Korea Polar Research Institute, 26 Songdomirae-ro, Yeonsu-gu, Incheon 21990, Republic of Korea.
Bioresources Collection & Research Division, Nakdonggang National Institute of Biological Resources, 137 Donam 2-gil, Sangju 37242, Republic of Korea.
Int J Syst Evol Microbiol. 2020 Apr;70(4):2912-2917. doi: 10.1099/ijsem.0.004122. Epub 2020 Mar 26.
A Gram-stain-negative, aerobic, yellow-pigmented, flexirubin-negative, rod-shaped and non-motile bacterial strain, PAMC 28998, was isolated from a surface sediment sample collected from the Canadian Beaufort Sea. Strain PAMC 28998 grew at 4-37 °C (optimum, 25 °C), at pH 7.0-9.0 (optimum, pH 7.5) and in the presence of 1.0-10.0 % (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain PAMC 28998 belongs to the genus showing the highest sequence similarity (96.8 %) with JB01H24. The average nucleotide identity and genome-to-genome distance values between PAMC 28998 and the most closely related species ( JB01H24) were 74.1 and 18.5 %, respectively, indicating that strain PAMC 28998 is clearly distinguished from . The genomic DNA G+C content calculated from genome sequences was 39.8 %. The major fatty acids (>10 %) were iso-C (19.5 %), anteiso-C (18.0 %), iso-C (11.6 %) and summed feature 3 (C ω6 and/or C ω7; 11.4 %). The major polar lipids were phosphatidylethanolamine, aminoglycolipid, two unidentified aminolipids, three unidentified phospholipids and four unidentified lipids. The major respiratory quinone was MK-6. Based on the phylogenetic, genomic and phenotypic data presented here, strain PAMC 28998 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed with the strain PAMC 28998 (=KCCM 43316 =JCM 33514).
一株革兰氏阴性、需氧、黄色、不含腐臭红、杆状、非运动的细菌菌株 PAMC 28998，是从加拿大波弗特海的一个表层沉积物样本中分离出来的。菌株 PAMC 28998 在 4-37°C（最佳温度 25°C）、pH 值 7.0-9.0（最佳 pH 值 7.5）和 1.0-10.0%（w/v）NaCl 的条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 PAMC 28998属于 属，与 JB01H24 的同源性最高（96.8%）。PAMC 28998 与最密切相关的物种（JB01H24）之间的平均核苷酸同一性和基因组到基因组的距离值分别为 74.1%和 18.5%，表明菌株 PAMC 28998与 属明显不同。从基因组序列计算得出的基因组 DNA G+C 含量为 39.8%。主要脂肪酸（>10%）为 iso-C（19.5%）、anteiso-C（18.0%）、iso-C（11.6%）和 sumed feature 3（C ω6 和/或 C ω7；11.4%）。主要极性脂为磷脂酰乙醇胺、氨基糖脂、两种未鉴定的氨基脂、三种未鉴定的磷脂和四种未鉴定的脂类。主要呼吸醌为 MK-6。根据本文提供的系统发育、基因组和表型数据，菌株 PAMC 28998被认为代表了属的一个新种，建议使用 PAMC 28998 株（=KCCM 43316 =JCM 33514）作为该新种的名称。
