MIVEGEC, Univ. Montpellier, CNRS, IRD, 911 avenue Agropolis, BP 64501, 34394 Montpellier cedex 5, France.
EID Méditerranée, 165 avenue Paul-Rimbaud, 34184 Montpellier Cedex 4, France.
Parasite. 2022;29:19. doi: 10.1051/parasite/2022020. Epub 2022 Mar 29.
The mosquito species Aedes (Ochlerotatus) coluzzii Rioux, Guilvard & Pasteur, 1998 was distinguished from its sibling species Aedes detritus (Haliday, 1833) using an isoenzymatic method that required the destruction of the entire specimen, therefore no holotype was designated by the species authors. We aimed to designate a neotype for Ae. coluzzii from specimens collected from the type-locality and individually reared up to adult stage. Genomic DNA was extracted from pupal exuvia and ITS2 was sequenced, enabling verification of the identity of each specimen as Ae. coluzzii or Ae. detritus. Among the series of Ae. coluzzii, a male was designated as neotype and deposited in a collection. To our knowledge, this is the first time the type of a mosquito species is deposited thanks to its molecular identification from its pupal exuvia. The set of identified specimens allowed additional phylogenetic and morphologic studies.
蚊种埃及伊蚊（白纹伊蚊）于 1998 年由 Rioux、Guilvard 和 Pasteur 从其近缘种埃及斑蚊（淡色库蚊）中区分出来，该方法使用同工酶方法，需要破坏整个标本，因此种作者未指定模式标本。我们的目的是从来自模式产地的标本中指定埃及伊蚊的近型，然后单独饲养至成虫阶段。从蛹蜕中提取基因组 DNA 并对 ITS2 进行测序，从而可以验证每个标本是埃及伊蚊还是埃及斑蚊。在一系列埃及伊蚊中，选择一只雄性作为近型并保存在一个收藏中。据我们所知，这是首次通过从蛹蜕的分子鉴定来保存蚊子物种的模式。这组鉴定的标本还允许进行额外的系统发育和形态学研究。
