School of Public Health, Shantou University, Shantou, Guangdong Province 515063, PR China; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China.
Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China.
Infect Genet Evol. 2022 Aug;102:105311. doi: 10.1016/j.meegid.2022.105311. Epub 2022 May 29.
Culicidae, the mosquito family, includes more than 3600 species subdivided into the subfamilies Anophelinae and Culicinae. One-third of mosquitoes belong to the Aedini tribe, which is subordinate to the subfamily Culicinae, which comprises common vectors of viral zoonoses. The tribe of Aedini is extremely diverse in morphology and geographical distribution and has high ecological and medical significance. However, knowledge about the systematics of the Aedini tribe is still limited owing to its large population and the similar morphological characteristics of its species. This study provides the first description of the complete mitochondrial (mt) genome sequence of Aedes vexans and Ochlerotatus caspius belonging to the Aedini tribe. The mt genomes of A. vexans and O. caspius are circular molecules that are 15,861 bp and 15,954 bp in size, with AT contents of 78.54% and 79.36%, respectively. Both the circular mt genomes comprise 37 functional subunits, including 13 protein-coding genes (PCGs), two ribosomal RNA genes, 22 transfer RNA genes (tRNAs), and a control region (also known as the AT-rich region). The most common start codons are ATT/ATG, apart from cox1 (TCG) and nad5 (GTG), while TAA is the termination codon for all PCGs. All tRNAs have a typical clover leaf structure, except tRNA Ser1. Phylogenetic analysis of the concatenated, aligned amino acid sequences of the 13 PCGs showed that A. vexans gathered with Aedes sp. in a sister taxon, and O. caspius gathered with Ochlerotatus sp. in a sister taxon. The findings from the present study support the concept of monophyly of all groups, ratify the current taxonomic classification, and provide vital molecular marker resources for further studies of the taxonomy, population genetics, and systematics of the Aedini tribe.
库蚊科（Culicidae）包括超过 3600 种蚊子，分为按蚊亚科（Anophelinae）和库蚊亚科（Culicinae）。三分之一的蚊子属于库蚊亚科的伊蚊族（Aedini），而该族是病毒人畜共患病的常见病媒。伊蚊族在形态和地理分布上具有极高的多样性，具有重要的生态和医学意义。然而，由于其庞大的种群和相似的物种形态特征，对该族的系统发育知识仍然有限。本研究首次描述了伊蚊族的致倦库蚊（Aedes vexans）和刺扰伊蚊（Ochlerotatus caspius）的完整线粒体（mt）基因组序列。致倦库蚊和刺扰伊蚊的 mt 基因组均为圆形分子，大小分别为 15861bp 和 15954bp，AT 含量分别为 78.54%和 79.36%。这两个圆形 mt 基因组均包含 37 个功能亚基，包括 13 个蛋白编码基因（PCGs）、两个核糖体 RNA 基因、22 个转移 RNA 基因（tRNAs）和一个控制区（也称为富含 AT 区）。最常见的起始密码子是 ATT/ATG，除了 cox1（TCG）和 nad5（GTG）外，所有 PCGs 的终止密码子都是 TAA。所有 tRNAs 均具有典型的三叶形结构，除了 tRNA Ser1。对 13 个 PCGs 串联、对齐氨基酸序列进行的系统发育分析表明，致倦库蚊与伊蚊属（Aedes sp.）聚为姊妹群，刺扰伊蚊与斑蚊属（Ochlerotatus sp.）聚为姊妹群。本研究结果支持所有分组的单系性概念，证实了当前的分类学分类，并为进一步研究伊蚊族的分类学、群体遗传学和系统发育提供了重要的分子标记资源。
