Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, School of Life Sciences, Lanzhou University, Tianshuinanlu #222, Lanzhou 730000, Gansu, China.
Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Kowloon, Hong Kong.
Food Funct. 2022 Apr 20;13(8):4537-4550. doi: 10.1039/d2fo00206j.
Selenium levels have a critical impact on livestock and poultry, and selenium nanoparticles (SeNPs) have shown significant efficiency in supplementation. This study identified a high-efficiency selenite reductase, SerV01, in LZ-01, which can convert SeO to SeNPs. Subsequently, SerV01 was introduced into the intestines of the broilers using the surface display-engineered Nissle 1917 (EcN). The results showed that the engineered bacteria (EcN-IS) significantly increased the selenium content by 0.87 mg kg, 0.52 mg kg, and 6.10 mg L in the liver, breast muscle, and serum, respectively. With SeNPs + EcN-IS treatment, glutathione peroxidase and thioredoxin reductase levels reached 0.7536 ± 0.03176 U μL protein and 2.463 ± 0.1685 U μL protein, respectively. With the modified probiotics, the proportion of beneficial intestinal flora increased, with and accounting for 75.85% and 0.19%. This technology provides a novel idea to facilitate the exploitation of selenium in broiler diets and improve antioxidant capability.
硒水平对家畜和家禽有重要影响，硒纳米颗粒（SeNPs）在补充方面表现出显著的效率。本研究在 LZ-01 中发现了一种高效的亚硒酸盐还原酶 SerV01，它可以将 SeO 转化为 SeNPs。随后，使用表面展示工程化的 Nissle 1917（EcN）将 SerV01 引入肉鸡的肠道。结果表明，工程菌（EcN-IS）分别使肝脏、胸肌和血清中的硒含量显著增加了 0.87mg/kg、0.52mg/kg 和 6.10mg/L。经 SeNPs+EcN-IS 处理后，谷胱甘肽过氧化物酶和硫氧还蛋白还原酶水平分别达到 0.7536±0.03176U·μL 蛋白和 2.463±0.1685U·μL 蛋白。通过改良益生菌，有益肠道菌群的比例增加， 和 分别占 75.85%和 0.19%。这项技术为开发肉鸡日粮中的硒并提高抗氧化能力提供了新的思路。
