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不同提取方法对竹笋膳食纤维的物化特性、结构特性及体外降血糖活性的比较。

Comparison of different extraction methods on the physicochemical, structural properties, and in vitro hypoglycemic activity of bamboo shoot dietary fibers.

机构信息

College of Food Science, Southwest University, Chongqing 400715, China; National Demonstration Center for Experimental Food Science and Technology Education (Southwest University), Chongqing 400715, China.

China National Bamboo Research Center, Hangzhou 310012, China.

出版信息

Food Chem. 2022 Aug 30;386:132642. doi: 10.1016/j.foodchem.2022.132642. Epub 2022 Mar 26.

DOI:10.1016/j.foodchem.2022.132642
PMID:35349899
Abstract

The effect of alkali extraction (AE), enzymatic extraction (EE), ultrasonic-assisted enzymatic extraction (UAEE), and shear homogeneous-assisted enzymatic extraction (SHAEE) on the physicochemical, structural properties, and in vitro hypoglycemic activity of bamboo shoot dietary fibers (BSDF) were investigated and compared. BSDF obtained by AE had the lowest protein content and crystallinity index. The lowest oil holding capacity (OHC) and highest protein content were observed in EE. BSDF generated highest OHC and glucose adsorption capacity by UAEE. SHAEE obtained the highest SDF content (17.89%), water-holding capacity (8.81 g/g), and α-amylase activity inhibition ratio (19.89%) and the smallest particle size (351.33 μm). BSDF extracted by SHAEE and UAEE presented a porous and loose structure. Furthermore, the in vitro hypoglycemic activity of the four BSDF samples generally followed the order of SHAEE > UAEE > EE > AE. Results show that SHAEE is an innovative and promising method to obtain BSDF with its excellent physicochemical and functional properties.

摘要

研究并比较了碱提取(AE)、酶提取(EE)、超声辅助酶提取(UAEE)和剪切均相辅助酶提取(SHAEE)对竹笋膳食纤维(BSDF)理化性质、结构特性和体外降血糖活性的影响。AE 得到的 BSDF 蛋白含量和结晶度指数最低。EE 得到的 BSDF 油保持能力(OHC)最低,蛋白含量最高。UAEE 得到的 BSDF 具有最高的 OHC 和葡萄糖吸附能力。SHAEE 得到的 SDF 含量(17.89%)、持水力(8.81g/g)和α-淀粉酶活性抑制率(19.89%)最高,粒径最小(351.33μm)。SHAEE 和 UAEE 提取的 BSDF 呈现多孔疏松的结构。此外,四种 BSDF 样品的体外降血糖活性通常遵循 SHAEE>UAEE>EE>AE 的顺序。结果表明,SHAEE 是一种获得具有优异理化性质和功能特性的 BSDF 的创新和有前途的方法。

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