Yeast Biofuel Group, DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, India.
Yeast Biofuel Group, DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, India.
Bioresour Technol. 2022 May;351:127067. doi: 10.1016/j.biortech.2022.127067. Epub 2022 Mar 26.
Dependency on fossil fuels raises an economic and ecological concern that has urged to look for alternative sources of energy. Bio-refinery concept is one of the alternate frameworks for the biomass conversion into biofuel and other value-added by-products. The present work illustrates importance of an oleaginous yeast Rhodotorula pacifica INDKK in an integrated bio-refinery field by utilizing renewable sugars generated from lignocellulosic biomass. The maximum 11.8 g/L lipid titer, 210.4 mg/L β-carotene and 7.1 g animal feed were produced by R. pacifica INDKK in bioreactor containing 5% (v/v) molasses supplemented with enzymatically hydrolyzed and alkali-pretreated sugarcane bagasse hydrolysate (35% v/v). Furthermore, xylooligosaccharides (20.6 g/L), a beneficial prebiotics were also produced from the hemicellulosic fraction separated after alkali pretreatment of bagasse. This novel concept of integrated yeast bio-refinery for concomitant production of biodiesel and multiple value-added products with minimum waste generation is proposed as a sustainable and profitable process.
对化石燃料的依赖引发了经济和生态方面的关注，促使人们寻求替代能源。生物炼制概念是将生物质转化为生物燃料和其他增值副产品的替代框架之一。本工作通过利用木质纤维素生物质产生的可再生糖，说明了产油酵母 Rhodotorula pacifica INDKK 在综合生物炼制领域的重要性。在含有 5%（v/v）糖蜜的生物反应器中，添加酶解和碱预处理的甘蔗渣水解物（35%v/v），R. pacifica INDKK 可生产出最高 11.8 g/L 的脂质浓度、210.4 mg/L 的β-胡萝卜素和 7.1 g 动物饲料。此外，从碱预处理后的蔗渣的半纤维素部分还可产生 20.6 g/L 的有益益生元木低聚糖。因此，提出了一种综合酵母生物炼制的新概念，可同时生产生物柴油和多种附加值产品，同时最大限度地减少废物产生，这是一种可持续且有利可图的工艺。
