Díaz-Ruiz Erick, Balbino Thércia R, Dos Santos Júlio C, Kumar Vinod, da Silva Silvio S, Chandel Anuj K
Department of Biotechnology, Engineering School of Lorena, University of São Paulo (EEL-USP), 12.602.810, Lorena, SP, Brazil.
School of Water, Energy and Environment, Cranfield University, Cranfield, MK43 0AL, UK.
Appl Biochem Biotechnol. 2024 Jul;196(7):4188-4204. doi: 10.1007/s12010-023-04761-x. Epub 2023 Nov 2.
Β-Carotene is a red-orange pigment that serves as a precursor to important pharmaceutical molecules like vitamin A and retinol, making it highly significant in the industrial sector. Consequently, there is an ongoing quest for more sustainable production methods. In this study, glucose and xylose, two primary sugars derived from sugarcane bagasse (SCB), were utilized as substrates for β-carotene production by Rhodotorula glutinis CCT-2186. To achieve this, SCB underwent pretreatment using NaOH, involved different concentrations of total solids (TS) (10%, 15%, and 20%) to remove lignin. Each sample was enzymatically hydrolyzed using two substrate loadings (5% and 10%). The pretreated SCB with 10%, 15%, and 20% TS exhibited glucose hydrolysis yields (%wt) of 93.10%, 91.88%, and 90.77%, respectively. The resulting hydrolysate was employed for β-carotene production under batch fermentation. After 72 h of fermentation, the SCB hydrolysate yielded a β-carotene concentration of 118.56 ± 3.01 mg/L. These findings showcase the robustness of R. glutinis as a biocatalyst for converting SCB into β-carotene.
β-胡萝卜素是一种红橙色色素，是维生素A和视黄醇等重要药物分子的前体，因此在工业领域具有重要意义。因此，人们一直在寻求更可持续的生产方法。在本研究中，葡萄糖和木糖这两种源自甘蔗渣（SCB）的主要糖类被用作粘红酵母CCT-2186生产β-胡萝卜素的底物。为此，SCB采用NaOH进行预处理，涉及不同浓度的总固体（TS）（10%、15%和20%）以去除木质素。每个样品使用两种底物负载量（5%和10%）进行酶水解。TS含量为10%、15%和20%的预处理SCB的葡萄糖水解产率（%wt）分别为93.10%、91.88%和90.77%。所得水解产物用于分批发酵生产β-胡萝卜素。发酵72小时后，SCB水解产物的β-胡萝卜素浓度为118.56±3.01mg/L。这些发现表明粘红酵母作为将SCB转化为β-胡萝卜素的生物催化剂具有强大的性能。
