[一种定量测定酵母菌落中乙醇氧化酶和过氧化氢酶的方法]
[A method of quantitative determination of alcohol oxidase and catalase in yeast colonies].
作者信息
Sibirnyĭ A A, Titorenko V I
出版信息
Ukr Biokhim Zh (1978). 1986 Sep-Oct;58(5):65-8.
PMID:3535190
Abstract
A rapid, simple and highly reproducible method is developed for qualitative determination of alcohol oxidase and catalase in yeast colonies using digitonin as a permeabilizing agent. The method permits finding differences in regulation of catalase by ethanol in methylotrophic and nonmethylotrophic yeasts.
摘要
开发了一种快速、简单且高度可重复的方法，以洋地黄皂苷作为通透剂，用于定性测定酵母菌落中的乙醇氧化酶和过氧化氢酶。该方法能够发现甲基营养型酵母和非甲基营养型酵母中乙醇对过氧化氢酶调节的差异。
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