Faculty of Natural Sciences, Department of Microbiology and Virology, Comenius University in Bratislava, Ilkovicova 6, 842 15, Bratislava, Slovak Republic.
Centre for Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38, Bratislava, Slovak Republic.
Folia Microbiol (Praha). 2022 Aug;67(4):641-647. doi: 10.1007/s12223-022-00968-3. Epub 2022 Mar 30.
KlUpc2p, a transcription factor belonging to the fungal binuclear cluster family, is an important regulator of ergosterol biosynthesis and azole drug resistance in Kluyveromyces lactis. In this work, we show that the absence of KlUpc2p generates Rag phenotype and modulates the K. lactis susceptibility to oxidants and calcofuor white. The KlUPC2 deletion leads to increased expression of KlMGA2 gene, encoding an important regulator of hypoxic and lipid biosynthetic genes in K. lactis and also KlHOG1 gene. The absence of KlUpc2p does not lead to statistically significant changes in glycerol, corroborating the expression of KlGPD1 gene, encoding NAD-dependent glycerol-3-phosphate dehydrogenase, that is similar in both the deletion mutant and the parental wild-type strain. Increased sensitivity of Klupc2 mutant cells to brefeldin A accompanied with significant increase in KlARF2 gene expression point to the involvement of KlUpc2p in intracellular signaling. Our observations highlight the connections between ergosterol and fatty acid metabolism to modulate membrane properties and point to the possible involvement of KlUpc2p in K. lactis oxidative stress response.
KlUpc2p 是一种真菌双核簇家族的转录因子，是酿酒酵母麦角固醇生物合成和唑类药物耐药性的重要调节剂。在这项工作中，我们表明 KlUpc2p 的缺失会产生 Rag 表型，并调节酿酒酵母对氧化剂和Calcofluor White 的敏感性。KlUPC2 的缺失导致 KlMGA2 基因的表达增加，该基因编码酿酒酵母缺氧和脂质生物合成基因的重要调节剂，也编码 KlHOG1 基因。KlUpc2p 的缺失不会导致甘油的表达发生统计学上显著的变化，这与编码 NAD 依赖性甘油-3-磷酸脱氢酶的 KlGPD1 基因的表达一致，该基因在缺失突变体和亲本野生型菌株中的表达相似。Klupc2 突变细胞对布雷菲德菌素 A 的敏感性增加伴随着 KlARF2 基因表达的显著增加，表明 KlUpc2p 参与了细胞内信号转导。我们的观察结果强调了麦角固醇和脂肪酸代谢之间的联系，以调节膜性质，并表明 KlUpc2p 可能参与了酿酒酵母的氧化应激反应。
