Faculty of Natural Sciences, Department of Microbiology and Virology, Comenius University in Bratislava, Ilkovicova 6, 842 15, Bratislava 4, Slovak Republic.
Folia Microbiol (Praha). 2019 Jul;64(4):579-586. doi: 10.1007/s12223-019-00682-7. Epub 2019 Jan 31.
In yeast, the STB5 gene encodes a transcriptional factor belonging to binuclear cluster class (ZnCys) of transcriptional regulators specific to ascomycetes. In this study, we prepared the Kluyveromyces lactis stb5Δ strain and assessed its responses to different stresses. We showed that KlSTB5 gene is able to complement the deficiencies of Saccharomyces cerevisiae stb5Δ mutant. The results of phenotypic analysis suggested that KlSTB5 gene deletion did not sensitize K. lactis cells to oxidative stress inducing compounds but led to Klstb5Δ resistance to 4-nitroquinoline-N-oxide and hygromycin B. Expression analysis indicated that the loss of KlSTB5 gene function induced the transcription of drug efflux pump encoding genes that might contribute to increased 4-nitroquinoline-N-oxide and hygromycin B tolerance. Our results show that KlStb5p functions as negative regulator of some ABC transporter genes in K. lactis.
在酵母中，STB5 基因编码一种属于二核簇类（ZnCys）的转录因子，是一种专门针对子囊菌的转录调节因子。在本研究中，我们制备了 Kluyveromyces lactis stb5Δ 菌株，并评估了其对不同应激的反应。我们表明，KlSTB5 基因能够弥补 Saccharomyces cerevisiae stb5Δ 突变体的缺陷。表型分析的结果表明，KlSTB5 基因缺失并没有使 K. lactis 细胞对氧化应激诱导化合物敏感，但导致 Klstb5Δ 对 4-硝基喹啉-N-氧化物和 Hygromycin B 的抗性。表达分析表明，KlSTB5 基因功能的丧失诱导了药物外排泵编码基因的转录，这可能有助于增加 4-硝基喹啉-N-氧化物和 Hygromycin B 的耐受性。我们的结果表明，KlStb5p 在 K. lactis 中作为一些 ABC 转运体基因的负调节因子发挥作用。
