Lourenço Bianca N, Coleman Amanda E, Berghaus Roy D, Tarigo Jaime L, Schmiedt Chad W, Brown Scott A
Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA.
Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA.
J Vet Intern Med. 2022 Mar;36(2):647-655. doi: 10.1111/jvim.16361. Epub 2022 Jan 19.
The role of the renin-angiotensin-aldosterone system in cats with chronic kidney disease (CKD) is incompletely understood.
To characterize components of the intrarenal renin-angiotensin system (RAS) in cats with CKD.
Eleven cats with naturally occurring CKD (CKD group) and 8 healthy control cats.
Renal tissue samples were evaluated by reverse-transcription polymerase chain reaction for renin, angiotensinogen, angiotensin-converting enzyme (ACE), and angiotensin II type 1 receptor transcript levels, and by liquid chromatography-mass spectrometry for quantification of angiotensin I, II, III, and IV concentrations. Linear mixed models were used to compare gene transcript levels and concentrations of angiotensin peptides between groups.
Cats of the CKD group were significantly older (P < .001) and more likely to be neutered (P = .007) than healthy control cats. Kidneys from cats with CKD had significantly higher transcript levels of angiotensinogen (P < .001) and lower transcript levels of ACE (P < .001) than those from control cats. Renal angiotensin I concentrations were increased in CKD compared with control kidneys (P = .001). No other significant differences in renal transcript levels or angiotensin peptide concentrations were noted between groups.
The intrarenal RAS might be activated in cats with CKD. Small sample size and differences in age, neuter status, and dietary sodium intake between groups might have limited the ability to identify a significant difference in concentration of renal angiotensin II.
肾素-血管紧张素-醛固酮系统在患有慢性肾病（CKD）的猫中的作用尚未完全明确。
描述患有CKD的猫肾内肾素-血管紧张素系统（RAS）的组成部分。
11只患有自然发生的CKD的猫（CKD组）和8只健康对照猫。
通过逆转录聚合酶链反应评估肾组织样本中肾素、血管紧张素原、血管紧张素转换酶（ACE）和血管紧张素II 1型受体的转录水平，并通过液相色谱-质谱法对血管紧张素I、II、III和IV的浓度进行定量。使用线性混合模型比较各组之间的基因转录水平和血管紧张素肽浓度。
与健康对照猫相比，CKD组的猫年龄显著更大（P <.001）且绝育的可能性更高（P =.007）。与对照猫的肾脏相比，患有CKD的猫的肾脏中血管紧张素原的转录水平显著更高（P <.001），而ACE的转录水平更低（P <.001）。与对照肾脏相比，CKD组的肾血管紧张素I浓度升高（P =.001）。各组之间在肾转录水平或血管紧张素肽浓度方面未发现其他显著差异。
患有CKD的猫的肾内RAS可能被激活。样本量小以及各组之间在年龄、绝育状态和饮食钠摄入量方面的差异可能限制了识别肾血管紧张素II浓度显著差异的能力。
