Huang Jane H C, Lourenço Bianca N, Schmiedt Chad W, Tarigo Jaime L, Coleman Amanda E
Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA.
Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA.
Physiol Rep. 2025 Jun;13(12):e70417. doi: 10.14814/phy2.70417.
Activities of the circulating and intrarenal renin-angiotensin(-aldosterone) systems (RA[A]S) are incompletely understood in people and cats with chronic kidney disease (CKD). We measured circulating and intrarenal RA(A)S markers in healthy cats (n = 8) and cats with induced CKD (n = 6 subjected to unilateral renal ischemia [RI group] and n = 5 subjected to RI and delayed contralateral nephrectomy [RI-DCN group]). Serum equilibrium concentrations of angiotensin peptides and aldosterone, plasma renin activity, and urinary aldosterone-to-creatinine ratio were evaluated before and after renal injury in CKD cats and at a single timepoint in healthy cats. Renal tissular concentrations of angiotensin peptides and mRNA levels of RA(A)S-related genes were measured in all cats. There was no significant correlation between circulating angiotensin peptide concentrations and their respective renal concentrations. Intrarenal angiotensin I concentrations and AGT transcript levels were positively correlated, and ACE transcript levels were negatively correlated with serum creatinine concentration. Circulating RA(A)S markers were not different between healthy and CKD groups, except for serum angiotensin 1-5, which was lower in the RI group compared to the healthy group. Intrarenal angiotensin peptide concentrations did not differ among groups. Compared to healthy cats, mRNA levels of ACE, AT1R, and REN were lower, and AGT levels were higher in one or both CKD group(s).
在患有慢性肾病（CKD）的人和猫中，循环和肾内肾素-血管紧张素（-醛固酮）系统（RA[A]S）的活动尚未完全明确。我们测量了健康猫（n = 8）以及诱导性CKD猫（n = 6只接受单侧肾缺血 [RI组]，n = 5只接受RI并延迟对侧肾切除术 [RI-DCN组]）的循环和肾内RA(A)S标志物。在CKD猫肾损伤前后以及健康猫的单个时间点评估了血管紧张素肽和醛固酮的血清平衡浓度、血浆肾素活性以及尿醛固酮与肌酐比值。测量了所有猫肾组织中血管紧张素肽的浓度以及RA(A)S相关基因的mRNA水平。循环血管紧张素肽浓度与其各自的肾浓度之间无显著相关性。肾内血管紧张素I浓度与AGT转录水平呈正相关，而ACE转录水平与血清肌酐浓度呈负相关。除血清血管紧张素1-5外，健康组和CKD组之间的循环RA(A)S标志物无差异，RI组的血清血管紧张素1-5低于健康组。各组之间肾内血管紧张素肽浓度无差异。与健康猫相比，在一个或两个CKD组中，ACE、AT1R和REN的mRNA水平较低，而AGT水平较高。
