Department of Oncology, The First Affiliated Hospital of Wannan Medical College, Wuhu, 241002, China.
Department of Clinical Medicine, Wannan Medical College, Wuhu, 241002, China.
World J Surg Oncol. 2022 Mar 30;20(1):101. doi: 10.1186/s12957-022-02500-w.
MicroRNAs play an important role in the genesis and progression of tumours, including colorectal cancer (CRC), which has a high morbidity and mortality rate. In this research, the role of miR-495-3p and HMGB1 in CRC was investigated.
We performed qRT-PCR to detect the expression of miR-495-3p in colorectal cancer tissues and cell lines. Functional experiments, such as CCK-8, EdU, Transwell and apoptosis assays, were conducted to explore the effects of miR-495-3p on the proliferation, migration and apoptosis of CRC cells in vitro. Then, database prediction, dual-luciferase reporter gene assays and functional experiments verified the role of the miR-495-3p target gene HMGB1 in CRC. Finally, rescue experiments were performed to investigate whether overexpression of HMGB1 could reverse the inhibitory effect of miR-495-3p on CRC cell proliferation in vivo and in vitro.
miR-495-3p was downregulated in colorectal cancer tissues and cell lines, inhibited the proliferation and migration of colorectal cancer cells and promoted cell apoptosis. Database prediction and dual-luciferase reporter gene assays showed that HMGB1 was the downstream target gene of miR-495-3p. We finally demonstrated that miR-495-3p inhibited CRC cell proliferation by targeting HMGB1 in vitro and in vivo.
Our research shows that miR-495-3p inhibits the progression of colorectal cancer by downregulating the expression of HMGB1, which indicates that miR-495-3p may become a potential therapeutic target for colorectal cancer.
微小 RNA 在肿瘤的发生和发展中起着重要作用，包括结直肠癌（CRC），其发病率和死亡率都很高。在这项研究中，研究了 miR-495-3p 和 HMGB1 在 CRC 中的作用。
我们通过 qRT-PCR 检测结直肠癌组织和细胞系中 miR-495-3p 的表达。进行 CCK-8、EdU、Transwell 和凋亡实验等功能实验，以研究 miR-495-3p 对 CRC 细胞体外增殖、迁移和凋亡的影响。然后，通过数据库预测、双荧光素酶报告基因实验和功能实验验证了 miR-495-3p 靶基因 HMGB1 在 CRC 中的作用。最后，进行了挽救实验，以研究 HMGB1 的过表达是否可以逆转 miR-495-3p 对 CRC 细胞在体内和体外增殖的抑制作用。
miR-495-3p 在结直肠癌组织和细胞系中下调，抑制结直肠癌细胞的增殖和迁移，促进细胞凋亡。数据库预测和双荧光素酶报告基因实验表明，HMGB1 是 miR-495-3p 的下游靶基因。我们最终证明 miR-495-3p 通过靶向 HMGB1 在体外和体内抑制 CRC 细胞增殖。
我们的研究表明，miR-495-3p 通过下调 HMGB1 的表达抑制结直肠癌的进展，这表明 miR-495-3p 可能成为结直肠癌的潜在治疗靶点。
