以泪液蛋白质组学作为一种有前途的结肠癌非侵入性筛查试验。
Proteomic profiling of tear fluid as a promising non-invasive screening test for colon cancer.
机构信息
Central Arkansas Veterans Healthcare System, 4300 W.7th, Little Rock, AR, 72205, USA; University of Arkansas for Medical Sciences, 4301 W. Markham, Little Rock, AR, 72205, USA.
University of Arkansas for Medical Sciences, 4301 W. Markham, Little Rock, AR, 72205, USA.
出版信息
Am J Surg. 2022 Jul;224(1 Pt A):19-24. doi: 10.1016/j.amjsurg.2022.03.029. Epub 2022 Mar 25.
BACKGROUND
Current screening options for colorectal cancer (CRC) are either invasive (colonoscopy) or have lower sensitivity to identify pre-malignant lesions (fecal immunochemical test). We proposed to identify protein profiles in tears of patients with both pre-malignant polyps and CRC; these profiles could have potential as a noninvasive screening test.
METHOD
Colonoscopy patients were divided into "high risk" group (CRC and tubular adenomatous polyp) and "low risk" (normal and hyperplastic polyps). Tear fluids from patients were analyzed by Liquid Chromatography Mass Spectrometry/Mass Spectrometry. The data were analyzed for protein expression, protein-protein interaction and gene set enrichment.
RESULTS
The results showed 80 proteins (18 up-regulated and 62 down-regulated) significantly differentiated in "high-risk" compared to "low-risk"; Twenty-eight of these show protein-protein interactions, 9 of which were associated with pathways demonstrated to be altered in CRC patients.
CONCLUSION
Our pilot data, though limited, demonstrated tear protein profiling could distinguish the groups of patients with and without colon lesions.
背景
目前用于结直肠癌（CRC）的筛查选择要么具有侵入性（结肠镜检查），要么对识别癌前病变的敏感性较低（粪便免疫化学试验）。我们提出在患有癌前息肉和 CRC 的患者的泪液中识别蛋白质谱；这些谱可能具有作为非侵入性筛查试验的潜力。
方法
将结肠镜检查患者分为“高危”组（CRC 和管状腺瘤性息肉）和“低危”组（正常和增生性息肉）。通过液相色谱-质谱/质谱分析患者的泪液。对蛋白质表达、蛋白质-蛋白质相互作用和基因集富集进行数据分析。
结果
结果显示，与“低危”相比，“高危”组中有 80 种蛋白质（18 种上调和 62 种下调）差异显著；其中 28 种显示蛋白质-蛋白质相互作用，其中 9 种与在 CRC 患者中显示改变的途径相关。
结论
尽管我们的初步数据有限，但表明泪液蛋白谱分析可以区分有无结肠病变的患者群体。
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