Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea.
Bio-MAX Institute, Seoul National University, Seoul, 08826, Republic of Korea.
Food Funct. 2022 Apr 20;13(8):4592-4599. doi: 10.1039/d1fo04362e.
Orobol, a metabolite of genistein, is rare in natural soybean. Several studies have revealed the immune-controlling effects of orobol on inflammatory diseases. Furthermore, a few studies have demonstrated that orobol decreases pro-inflammatory compounds resulting in the alleviation of allergic reactions. However, the relationship between orobol and atopic dermatitis (AD) in animal models has not been revealed. Therefore, we sought to investigate the effects of orobol on AD-like symptoms. AD-like symptoms and skin lesions were induced by repeated topical application of extract (DFE) on the skin of NC/Nga mice. Topical application of orobol attenuated DFE-induced AD-like symptoms and transepidermal water loss and increased skin hydration. Histopathological analysis revealed that orobol alleviated DFE-induced eosinophil and mast cell infiltration into the skin. These observations occurred concomitantly with the downregulation of inflammatory markers including serum TARC, MDC, and IgE. In addition, orobol alleviated dorsal Th2 cytokines such as IL-4 and IL-13. Pre-treatment of orobol decreased the activity of the MAPKs and NF-κB signalling cascade in the TNFα/IFNγ-induced HaCaT cell line. These results suggest that orobol, a natural dietary isoflavone, has therapeutic efficacy for the prevention and treatment of AD.
黄豆苷元的代谢产物黄豆黄苷在天然大豆中较为罕见。有几项研究揭示了黄豆黄苷对炎症性疾病的免疫控制作用。此外，有几项研究表明，黄豆黄苷可减少促炎化合物，从而缓解过敏反应。然而，动物模型中黄豆黄苷与特应性皮炎（AD）之间的关系尚未被揭示。因此，我们试图研究黄豆黄苷对 AD 样症状的影响。通过在 NC/Nga 小鼠皮肤上反复涂抹提取物（DFE）诱导 AD 样症状和皮肤损伤。黄豆黄苷的局部应用减轻了 DFE 诱导的 AD 样症状和经表皮水分流失，并增加了皮肤水分。组织病理学分析显示，黄豆黄苷减轻了 DFE 诱导的嗜酸性粒细胞和肥大细胞浸润到皮肤中。这些观察结果与包括血清 TARC、MDC 和 IgE 在内的炎症标志物的下调同时发生。此外，黄豆黄苷减轻了背部 Th2 细胞因子如 IL-4 和 IL-13 的产生。黄豆黄苷预处理降低了 TNFα/IFNγ诱导的 HaCaT 细胞系中 MAPKs 和 NF-κB 信号级联的活性。这些结果表明，天然膳食异黄酮黄豆黄苷具有预防和治疗 AD 的治疗功效。
