Emilio Cendejas Bueno, Clinical Microbiology Department, Hospital La Paz, Madrid, Paseo de La Castellana 261, 28046 Madrid, Spain.
Rev Esp Quimioter. 2022 Jun;35(3):284-287. doi: 10.37201/req/164.2021. Epub 2022 Mar 31.
Bloodstream infections (BSI) caused by extended-spectrum beta-lactamases Enterobacteriaceae (ESBL-E) are associated with high rates of treatment failure and increased mortality, especially when appropriate antimicrobial therapy is delayed. Our aim was to evaluate the anticipation of ESBLs detection and the potential improvement of the time response of the Vitek 2 System (BioMérieux; France).
We compared this lateral flow immunoassay when used directly on fluid from positive blood cultures to the VITEK2 AST system. We evaluated 80 isolates, 61 tested directly on fluid from positive blood cultures and 19 tested on fluid from blood cultures spiked with known ESBL positive and negative organisms.
The concordance between the CTX-LFIA and the reference method (Vitek 2) had a Cohen´s Kappa coefficient of 0.97, indicating a particularly good correlation between both compared methods.
This lateral flow immunoassay can be more rapid than the Vitek 2 for earlier presumptive identification of CTX-M ESBLs and can be useful to anticipate results and the adjustment of antimicrobial therapy.
产超广谱β-内酰胺酶肠杆菌科（ESBL-E）引起的血流感染（BSI）与治疗失败率高和死亡率增加相关，尤其是在适当的抗菌治疗延迟时。我们的目的是评估 ESBL 检测的预期，并可能提高 Vitek 2 系统（法国生物梅里埃公司）的时间反应。
我们将直接用于阳性血培养物液体的侧向流动免疫测定与 VITEK2 AST 系统进行了比较。我们评估了 80 株分离株，61 株直接在阳性血培养物的液体中检测，19 株在已知 ESBL 阳性和阴性生物体的血培养物液体中检测。
CTX-LFIA 与参考方法（Vitek 2）之间的一致性具有 Cohen's Kappa 系数为 0.97，表明两种比较方法之间具有特别好的相关性。
这种侧向流动免疫测定比 Vitek 2 更快，可以更早地推测 CTX-M ESBL 的存在，并有助于预测结果和调整抗菌治疗。
