Oil Crops Research Institute, Hubei Key Laboratory of Lipid Chemistry and Nutrition, and Key Laboratory of Oilseeds Processing, Ministry of Agriculture, Chinese Academy of Agricultural Science, Wuhan, China.
Wuhan Polytechnic University, Wuhan, China.
J Sci Food Agric. 2022 Sep;102(12):5495-5501. doi: 10.1002/jsfa.11904. Epub 2022 Apr 15.
The INFOGEST model is a standardized general in vitro digestion study, but it cannot accurately simulate the fatty acid release process of lipids in the stomach and small intestine. In this study, the internationally universal INFOGEST 2019 was used as the basic model and flaxseed oil emulsion was used as the research object. In various improvement models, the effect of fatty acid release rate on the oxidation stability of flaxseed oil was assessed by adding rabbit stomach extract and changing the order of bile salts addition.
With the presence of rabbit gastric extract, flaxseed oil emulsion flocculation and coalescence in stomach were reduced, and the absolute value of ζ-potential increased. Moreover, the release rate of fatty acids in the small intestine increased by 12.14%. The amount of lipid oxidation product (i.e. hexanal) in the gastric and intestinal phases increased by 0.08 ppb. In addition, the fatty acid release rate in the small intestine phase increased by 5.85% and the hexanal content increased by 0.011 ppb in the digestion model of adding bile salts before adjusting the pH in the small intestine phase compared with the model of adjusting the pH first and then adding bile salts.
The results obtained from this study will contribute to finding the most suitable static digestion model for simulating digestion and oxidation of lipid during lipid gastrointestinal digestion. © 2022 Society of Chemical Industry.
INFOGEST 模型是一种标准化的通用体外消化研究模型，但无法准确模拟胃和小肠中脂质的脂肪酸释放过程。在本研究中，使用国际通用的 INFOGEST 2019 作为基础模型，以亚麻籽油乳液为研究对象。在各种改进模型中，通过添加兔胃提取物和改变胆盐添加顺序，评估脂肪酸释放率对亚麻籽油氧化稳定性的影响。
有兔胃提取物存在时，胃中亚麻籽油乳液的絮凝和聚结减少，ζ-电位绝对值增加。此外，小肠中脂肪酸的释放速率增加了 12.14%。胃和肠相中脂质氧化产物（即己醛）的含量增加了 0.08 ppb。此外，与先调节小肠相 pH 值后添加胆盐的模型相比，在小肠相添加胆盐前调节 pH 值的消化模型中，脂肪酸在小肠相的释放速率增加了 5.85%，己醛含量增加了 0.011 ppb。
本研究的结果将有助于找到最适合模拟脂质胃肠道消化过程中消化和氧化的静态消化模型。© 2022 英国化学学会。
