Morisette Jeffrey, Burgiel Stanley, Brantley Kelsey, Daniel Wesley M, Darling John, Davis Jeanette, Franklin Thomas, Gaddis Keith, Hunter Margaret, Lance Richard, Leskey Tracy, Passamaneck Yale, Piaggio Antoinette, Rector Brian, Sepulveda Adam, Smith Melissa, Stepien Carol A, Wilcox Taylor
National Invasive Species Council Staff, Department of the Interior, Office of the Secretary, USA.
U.S. Geological Survey Wetland and Aquatic Research Center, 7920 NW 71 St. Gainesville, FL 32653, USA.
Manag Biol Invasion. 2021 Jul 9;12(3):747-775. doi: 10.3391/mbi.2021.12.3.15.
Invasive species surveillance programs can utilize environmental DNA sampling and analysis to provide information on the presence of invasive species. Wider utilization of eDNA techniques for invasive species surveillance may be warranted. This paper covers topics directed towards invasive species managers and eDNA practitioners working at the intersection of eDNA techniques and invasive species surveillance. It provides background information on the utility of eDNA for invasive species management and points to various examples of its use across federal and international programs. It provides information on 1) why an invasive species manager should consider using eDNA, 2) deciding if eDNA can help with the manager's surveillance needs, 3) important components to operational implementation, and 4) a high-level overview of the technical steps necessary for eDNA analysis. The goal of this paper is to assist invasive species managers in deciding if, when, and how to use eDNA for surveillance. If eDNA use is elected, the paper provides guidance on steps to ensure a clear understanding of the strengths and limitation of the methods and how results can be best utilized in the context of invasive species surveillance.
入侵物种监测计划可以利用环境DNA采样和分析来提供有关入侵物种存在情况的信息。可能有必要更广泛地利用环境DNA技术进行入侵物种监测。本文涵盖了针对在环境DNA技术与入侵物种监测交叉领域工作的入侵物种管理人员和环境DNA从业者的主题。它提供了关于环境DNA在入侵物种管理中的效用的背景信息，并指出了其在联邦和国际计划中的各种使用实例。它提供了以下方面的信息：1）入侵物种管理人员为何应考虑使用环境DNA；2）确定环境DNA是否有助于满足管理人员的监测需求；3）业务实施的重要组成部分；4）环境DNA分析所需技术步骤的概述。本文的目的是协助入侵物种管理人员决定是否、何时以及如何使用环境DNA进行监测。如果选择使用环境DNA，本文将提供相关步骤的指导，以确保清楚了解这些方法的优势和局限性，以及如何在入侵物种监测的背景下最佳利用结果。
