Department of Urology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250021, China.
Department of Urology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, China.
Oxid Med Cell Longev. 2022 Mar 21;2022:8151917. doi: 10.1155/2022/8151917. eCollection 2022.
Nitric oxide synthase- (NOS-) dependent endothelial dysfunction induced by oxidative stress (OS) is assumed to play a pivotal role in the pathogenesis and progression of diabetes mellitus-related erectile dysfunction (DMED). Cysteine-rich whey protein isolate (CR-WPI) is a widely used protein supplement and has been confirmed to reduce reactive oxygen species (ROS) by increasing cellular antioxidant glutathione (GSH). However, it is currently unknown whether CR-WPI elicits therapeutic effects in DMED. Here, we provide diabetic rats with CR-WPI to determine its effect on DMED and the underlying mechanisms. The results suggest that CR-WPI supplementation increased GSH biosynthesis and reduced ROS content and simultaneously upregulated the dimethylarginine dimethylaminohydrolase (DDAH)/asymmetrical dimethylarginine (ADMA)/nitric oxide synthase (NOS) metabolic pathway. Evaluation of intracavernous pressure (ICP) also showed an improvement of penile erectile function in CR-WPI-treated rats. The results of the vitro cell culture showed that glutathione pretreatment protected corpus cavernosum smooth muscle cells (CCSMC) from HO-induced apoptosis by decreasing Caspase 9 and Caspase 3 expressions. These results augur well for the potential therapeutic application of dietary CR-WPI supplementation for treating diabetic erectile dysfunction.
一氧化氮合酶-（NOS-）依赖的内皮功能障碍由氧化应激（OS）引起，被认为在糖尿病相关勃起功能障碍（DMED）的发病机制和进展中起关键作用。富含半胱氨酸的乳清蛋白分离物（CR-WPI）是一种广泛使用的蛋白质补充剂，已被证实通过增加细胞抗氧化谷胱甘肽（GSH）来减少活性氧（ROS）。然而，目前尚不清楚 CR-WPI 是否会对 DMED 产生治疗作用。在这里，我们给糖尿病大鼠提供 CR-WPI，以确定其对 DMED 的影响及其潜在机制。结果表明，CR-WPI 补充剂增加了 GSH 的生物合成，降低了 ROS 含量，同时上调了二甲基精氨酸二甲氨基水解酶（DDAH）/不对称二甲基精氨酸（ADMA）/一氧化氮合酶（NOS）代谢途径。对阴茎海绵体压（ICP）的评估也表明，CR-WPI 治疗的大鼠阴茎勃起功能得到改善。体外细胞培养的结果表明，谷胱甘肽预处理通过降低 Caspase 9 和 Caspase 3 的表达来保护海绵体平滑肌细胞（CCSMC）免受 HO 诱导的凋亡。这些结果预示着饮食补充 CR-WPI 治疗糖尿病性勃起功能障碍具有潜在的治疗应用前景。
